Validation of multiplexed human papillomavirus serology using pseudovirions bound to heparin-coated beads

• Published in: Journal of general virology Vol. 91; no. 7; pp. 1840 - 1848
• Main Authors: Faust, H., Knekt, P., Forslund, O., Dillner, J.
• Format: Journal Article
• Language: English
• Published: England 01.07.2010
• Subjects: Adult; Alphapapillomavirus - classification; Alphapapillomavirus - immunology; Animals; Basic Medicine; Cell Line; Child; DNA, Viral - classification; DNA, Viral - immunology; Female; Heparin; Human papillomavirus; Humans; Immunoassay - instrumentation; Immunoassay - methods; Male; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Microbiology in the Medical Area; Middle Aged; Mikrobiologi inom det medicinska området; Reproducibility of Results; Sensitivity and Specificity; Serologic Tests - instrumentation; Serologic Tests - methods; Uterine Cervical Neoplasms - virology

This study developed and validated a high-throughput human papillomavirus (HPV) serology method based on Luminex technology, using pseudovirions (PsVs) of eight mucosal HPV types (HPV-6, -11, -16, -18, -31, -45, -52 and -58) and two cutaneous HPV types (HPV-5 and -38) bound to heparin-coated beads....