Identification of delta/notch-like epidermal growth factor-related receptor as the Tr antigen in paraneoplastic cerebellar degeneration

• Published in: Annals of neurology Vol. 71; no. 6; pp. 815 - 824
• Main Authors: de Graaff, Esther, Maat, Peter, Hulsenboom, Esther, van den Berg, Robert, van den Bent, Martin, Demmers, Jeroen, Lugtenburg, Pieternella J., Hoogenraad, Casper C., Sillevis Smitt, Peter
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2012; Wiley-Liss; Wiley Subscription Services, Inc
• Subjects: Adult; Aged; Animals; Autoantibodies - blood; beta-Galactosidase - metabolism; Biological and medical sciences; Cell Line, Transformed; Child; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Epidermal growth factor; Female; Glycosylation; Hippocampus - pathology; Hodgkin Disease - immunology; Humans; Male; Mass Spectrometry; Medical research; Medical sciences; Middle Aged; Nerve Tissue Proteins - immunology; Nerve Tissue Proteins - metabolism; Neurology; Neurons - metabolism; Paraneoplastic Cerebellar Degeneration - immunology; Paraneoplastic Cerebellar Degeneration - metabolism; Rats; Receptors, Cell Surface - immunology; Receptors, Cell Surface - metabolism; Rodents; Tandem Repeat Sequences - immunology; Young Adult; Cerebellum; Nervous system diseases; Growth factor receptor; Epidermal growth factor; Central nervous system; Degeneration; Paraneoplastic syndrome; Encephalon

Objective: Anti‐Tr is among the better described autoantibodies in paraneoplastic cerebellar degeneration (PCD) combined with Hodgkin lymphoma (HL); however, the Tr antigen remains unidentified. Methods: We used immunoprecipitation of total rat brain extract followed by mass spectrometry to identify...