An infectivity-enhancing site on the SARS-CoV-2 spike protein targeted by antibodies

• Published in: Cell Vol. 184; no. 13; pp. 3452 - 3466.e18
• Main Authors: Liu, Yafei, Soh, Wai Tuck, Kishikawa, Jun-ichi, Hirose, Mika, Nakayama, Emi E., Li, Songling, Sasai, Miwa, Suzuki, Tatsuya, Tada, Asa, Arakawa, Akemi, Matsuoka, Sumiko, Akamatsu, Kanako, Matsuda, Makoto, Ono, Chikako, Torii, Shiho, Kishida, Kazuki, Jin, Hui, Nakai, Wataru, Arase, Noriko, Nakagawa, Atsushi, Matsumoto, Maki, Nakazaki, Yukoh, Shindo, Yasuhiro, Kohyama, Masako, Tomii, Keisuke, Ohmura, Koichiro, Ohshima, Shiro, Okamoto, Toru, Yamamoto, Masahiro, Nakagami, Hironori, Matsuura, Yoshiharu, Kato, Takayuki, Okada, Masato, Standley, Daron M., Shioda, Tatsuo, Arase, Hisashi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.06.2021
• Subjects: ADE; angiotensin converting enzyme 2; Animals; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; antibody-dependent enhancement; Cell Line; Chlorocebus aethiops; COVID-19 - immunology; COVID-19 infection; cryo-electron microscopy; cryo-EM; docking model; HEK293 Cells; Humans; mutational analysis; pathogenicity; Protein Binding - immunology; Protein Domains - immunology; SARS-CoV-2 - immunology; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; Vero Cells; ADE; docking model; antibody-dependent enhancement; angiotensin converting enzyme 2; cryo-EM; cryo-electron microscopy

Antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein prevent SARS-CoV-2 infection. However, the effects of antibodies against other spike protein domains are largely unknown. Here, we screened a series of anti-spike monoclonal antibodies from coronavirus disease 2019...