Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 66; no. 4; pp. 549 - 555
• Main Authors: Chu, Daniel K W, Pan, Yang, Cheng, Samuel M S, Hui, Kenrie P Y, Krishnan, Pavithra, Liu, Yingzhi, Ng, Daisy Y M, Wan, Carrie K C, Yang, Peng, Wang, Quanyi, Peiris, Malik, Poon, Leo L M
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.2020
• Subjects: Assaying; Betacoronavirus - genetics; Clinical Laboratory Techniques; Coronavirus Infections - diagnosis; Coronavirus Infections - epidemiology; Coronavirus Infections - virology; Coronaviruses; COVID-19; COVID-19 Testing; Deoxyribonucleic acid; Detection limits; Disease Outbreaks; Disease transmission; DNA; Epidemics; Genomes; Health care; Humans; Medical diagnosis; Medical personnel; Outbreaks; Pandemics; Patients; Phylogeny; Plasmids; Pneumonia, Viral - diagnosis; Pneumonia, Viral - epidemiology; Pneumonia, Viral - virology; Real-Time Polymerase Chain Reaction; Respiratory diseases; RNA, Viral - genetics; RNA, Viral - isolation & purification; SARS-CoV-2; Severe acute respiratory syndrome; Viral diseases; Viruses; China

Abstract Background A novel coronavirus of zoonotic origin (2019-nCoV) has recently been identified in patients with acute respiratory disease. This virus is genetically similar to SARS coronavirus and bat SARS-like coronaviruses. The outbreak was initially detected in Wuhan, a major city of China,...