UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner

• Published in: Biochemical and biophysical research communications Vol. 527; no. 3; pp. 668 - 675
• Main Authors: Cho, Jaeyong, Hiramoto, Masaki, Masaike, Yuka, Sakamoto, Satoshi, Imai, Yoichi, Imai, Yumi, Handa, Hiroshi, Imai, Takeshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.06.2020
• Subjects: Animals; Arginine; Arginine - metabolism; Cells, Cultured; Endoplasmic; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Glucosyltransferases - metabolism; Golgi apparatus; HEK293 Cells; Humans; Insulin; Insulin Secretion; Insulin-Secreting Cells - metabolism; magnetism; Male; Mice; Mice, Transgenic; Models, Molecular; proinsulin; Proinsulin - metabolism; Reticulum; UGGT1; Reticulum; Arginine; Insulin; UGGT1; Endoplasmic

We sought to clarify a pathway by which L- and dD-arginine simulate insulin secretion in mice and cell lines and obtained the following novel two findings. (1) Using affinity magnetic nanobeads technology, we identified that proinsulin is retained in the endoplasmic reticulum (ER) through UDP-glucos...