High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity

• Published in: Cell Vol. 179; no. 7; pp. 1636 - 1646.e15
• Main Authors: Setliff, Ian, Shiakolas, Andrea R., Pilewski, Kelsey A., Murji, Amyn A., Mapengo, Rutendo E., Janowska, Katarzyna, Richardson, Simone, Oosthuysen, Charissa, Raju, Nagarajan, Ronsard, Larance, Kanekiyo, Masaru, Qin, Juliana S., Kramer, Kevin J., Greenplate, Allison R., McDonnell, Wyatt J., Graham, Barney S., Connors, Mark, Lingwood, Daniel, Acharya, Priyamvada, Morris, Lynn, Georgiev, Ivelin S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.12.2019
• Subjects: antibodies; Antibodies, Neutralizing - chemistry; Antibodies, Neutralizing - immunology; antibody discovery; antibody repertoire; antigens; Antigens - chemistry; Antigens - immunology; B cells; B-lymphocytes; broadly neutralizing antibodies; broadly neutralizing antibody; Cells, Cultured; DNA barcoding; Epitope Mapping - methods; Epitopes - chemistry; Epitopes - immunology; HEK293 Cells; high-throughput nucleotide sequencing; High-Throughput Nucleotide Sequencing - methods; High-Throughput Screening Assays - methods; HIV; HIV Antibodies - chemistry; HIV Antibodies - immunology; HIV infections; Human immunodeficiency virus; Humans; immune response; influenza; Receptors, Antigen, B-Cell - chemistry; Receptors, Antigen, B-Cell - immunology; Sequence Analysis, DNA - methods; single cell immunology; Single-Cell Analysis - methods; systems immunology; THP-1 Cells; vaccination; vaccine development; influenza; broadly neutralizing antibody; single cell immunology; HIV; antibody discovery; antibodies; B cells; systems immunology; antibody repertoire

B cell receptor (BCR) sequencing is a powerful tool for interrogating immune responses to infection and vaccination, but it provides limited information about the antigen specificity of the sequenced BCRs. Here, we present LIBRA-seq (linking B cell receptor to antigen specificity through sequencing)...