An optimized method for measuring fatty acids and cholesterol in stable isotope-labeled cells

• Published in: Journal of lipid research Vol. 58; no. 2; pp. 460 - 468
• Main Authors: Argus, Joseph P., Yu, Amy K., Wang, Eric S., Williams, Kevin J., Bensinger, Steven J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2017; Journal of Lipid Research; The American Society for Biochemistry and Molecular Biology; Elsevier
• Subjects: Cell Line; Cholesterol; Cholesterol - isolation & purification; Cholesterol - metabolism; Contamination; Data processing; Fatty acids; Fatty Acids - isolation & purification; Fatty Acids - metabolism; Humans; Isotope Labeling - methods; Isotopes - pharmacology; Lipid Metabolism; Lipids; lipids/chemistry; mass spectrometry; Methods; neutral lipids; Quantitation; sphingolipids; stable isotope labeling; mass spectrometry; stable isotope labeling; lipids/chemistry; sphingolipids; neutral lipids

Stable isotope labeling has become an important methodology for determining lipid metabolic parameters of normal and neoplastic cells. Conventional methods for fatty acid and cholesterol analysis have one or more issues that limit their utility for in vitro stable isotope-labeling studies. To addres...