Evaluation of chromosomal insertion loci in the Pseudomonas putida KT2440 genome for predictable biosystems design

• Published in: Metabolic engineering communications Vol. 11; no. C; p. e00139
• Main Authors: Chaves, Julie E., Wilton, Rosemarie, Gao, Yuqian, Munoz, Nathalie Munoz, Burnet, Meagan C., Schmitz, Zachary, Rowan, John, Burdick, Leah H., Elmore, Joshua, Guss, Adam, Close, Dan, Magnuson, Jon K., Burnum-Johnson, Kristin E., Michener, Joshua K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2020; Elsevier
• Subjects: BASIC BIOLOGICAL SCIENCES; fluorescence; fluorescent proteins; Full Length; gene expression; genes; genomics; loci; metabolic engineering; phenotype; protein synthesis; proteomics; Pseudomonas putida; synthetic biology; viability
• ISSN: 2214-0301; 2214-0301
• DOI: 10.1016/j.mec.2020.e00139

The development of Pseudomonas strains for industrial production of fuels and chemicals will require the integration of heterologous genes and pathways into the chromosome. Finding the most appropriate integration site to maximize strain performance is an essential part of the strain design process....