Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types

• Published in: Cell Vol. 166; no. 2; pp. 451 - 467
• Main Authors: Loh, Kyle M., Chen, Angela, Koh, Pang Wei, Deng, Tianda Z., Sinha, Rahul, Tsai, Jonathan M., Barkal, Amira A., Shen, Kimberle Y., Jain, Rajan, Morganti, Rachel M., Shyh-Chang, Ng, Fernhoff, Nathaniel B., George, Benson M., Wernig, Gerlinde, Salomon, Rachel E.A., Chen, Zhenghao, Vogel, Hannes, Epstein, Jonathan A., Kundaje, Anshul, Talbot, William S., Beachy, Philip A., Ang, Lay Teng, Weissman, Irving L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.07.2016
• Subjects: Bone and Bones - cytology; Bone and Bones - metabolism; Bone Morphogenetic Proteins - metabolism; bones; chromatin; gene expression regulation; heart; Heart - growth & development; Homeodomain Proteins - metabolism; Humans; Mesoderm - cytology; Mesoderm - metabolism; muscles; Myocytes, Cardiac - metabolism; Pluripotent Stem Cells - metabolism; Primitive Streak - cytology; Primitive Streak - metabolism; Signal Transduction; Single-Cell Analysis; Somites - metabolism; Stem Cells; Tumor Suppressor Proteins - metabolism; Wnt Proteins - antagonists & inhibitors; Wnt Proteins - metabolism

Stem-cell differentiation to desired lineages requires navigating alternating developmental paths that often lead to unwanted cell types. Hence, comprehensive developmental roadmaps are crucial to channel stem-cell differentiation toward desired fates. To this end, here, we map bifurcating lineage c...