The Vanadium Iodoperoxidase from the marine flavobacteriaceae species Zobellia galactanivorans reveals novel molecular and evolutionary features of halide specificity in the vanadium haloperoxidase enzyme family

• Published in: Applied and Environmental Microbiology Vol. 80; no. 24; pp. 7561 - 7573
• Main Authors: Fournier, Jean-Baptiste, Rebuffet, Etienne, Delage, Ludovic, Grijol, Romain, Meslet-Cladière, Laurence, Rzonca, Justyna, Potin, Philippe, Michel, Gurvan, Czjzek, Mirjam, Leblanc, Catherine
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.12.2014
• Subjects: Amino Acid Sequence; Amino acids; Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Chemical elements; Crystals; Curvularia inaequalis; Enzymes; Enzymology and Protein Engineering; Escherichia coli; Evolution, Molecular; Evolutionary biology; Flavobacteriaceae; Flavobacteriaceae - classification; Flavobacteriaceae - enzymology; Flavobacteriaceae - genetics; Flavobacteriaceae - isolation & purification; Flavobacterium; Iodide Peroxidase - chemistry; Iodide Peroxidase - genetics; Iodide Peroxidase - metabolism; Kinetics; Models, Molecular; Molecular biology; Molecular Sequence Data; Multigene Family; Peroxidase - chemistry; Peroxidase - genetics; Peroxidase - metabolism; Phylogeny; Seawater - microbiology; Sequence Alignment; Streptomyces; Substrate Specificity; Vanadium - metabolism; Zobellia

Vanadium haloperoxidases (VHPO) are key enzymes that oxidize halides and are involved in the biosynthesis of organo-halogens. Until now, only chloroperoxidases (VCPO) and bromoperoxidases (VBPO) have been characterized structurally, mainly from eukaryotic species. Three putative VHPO genes were pred...