Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 32; pp. 13499 - 13504
• Main Authors: Felgner, Philip L, Kayala, Matthew A, Vigil, Adam, Burk, Chad, Nakajima-Sasaki, Rie, Pablo, Jozelyn, Molina, Douglas M, Hirst, Siddiqua, Chew, Janet S.W, Wang, Dongling, Tan, Gladys, Duffield, Melanie, Yang, Ron, Neel, Julien, Chantratita, Narisara, Bancroft, Greg, Lertmemongkolchai, Ganjana, Davies, D. Huw, Baldi, Pierre, Peacock, Sharon, Titball, Richard W
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 11.08.2009; National Acad Sciences
• Subjects: Antibodies; Antigens; Antigens, Bacterial - classification; Antigens, Bacterial - immunology; Bacteria; Bacterial proteins; Bacterial Proteins - immunology; Biological Sciences; Burkholderia pseudomallei; Burkholderia pseudomallei - immunology; Case-Control Studies; Cross Reactions - immunology; Diagnostic tests; Epitope Mapping; Humans; Immune system; Infections; Melioidosis; Melioidosis - diagnosis; Melioidosis - immunology; Molecules; Protein Array Analysis; Proteomes; Reactivity; Serologic Tests; Singapore; Thailand; Tropical medicine; United States; Vaccination; Thailand; United States; Singapore; United States > US; USA

Understanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. To provide insight into this area, we fabricated a protein microarray containing 1,205 Burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera...