Biochemical characterization of SARS-CoV-2 nucleocapsid protein

• Published in: Biochemical and biophysical research communications Vol. 527; no. 3; pp. 618 - 623
• Main Authors: Zeng, Weihong, Liu, Guangfeng, Ma, Huan, Zhao, Dan, Yang, Yunru, Liu, Muziying, Mohammed, Ahmed, Zhao, Changcheng, Yang, Yun, Xie, Jiajia, Ding, Chengchao, Ma, Xiaoling, Weng, Jianping, Gao, Yong, He, Hongliang, Jin, Tengchuan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.06.2020; Published by Elsevier Inc
• Subjects: ambient temperature; Antibodies, Viral - blood; Antigenicity; antigens; Betacoronavirus - chemistry; chromatography; Coronavirus Infections; COVID-19; COVID-19 infection; diagnostic techniques; fluorescence; Humans; immunity; Nucleic Acids; nucleocapsid; Nucleocapsid protein; nucleocapsid proteins; Nucleocapsid Proteins - chemistry; Pandemics; Pneumonia, Viral; Protein Binding; Protein Multimerization; Protein Structure, Tertiary; RNA packaging; SARS-CoV-2; SAXS; Scattering, Small Angle; Severe acute respiratory syndrome coronavirus 2; small-angle X-ray scattering; Structure and function; virion; X-Ray Diffraction; COVID-19; SARS-CoV-2; Antigenicity; Nucleocapsid protein; Structure and function; SAXS

The nucleocapsid (N) protein is an important antigen for coronavirus, which participate in RNA package and virus particle release. In this study, we expressed the N protein of SARS-CoV-2 and characterized its biochemical properties. Static light scattering, size exclusive chromatography, and small-a...