HIV-1 Integration Landscape during Latent and Active Infection

• Published in: Cell Vol. 160; no. 3; pp. 420 - 432
• Main Authors: Cohn, Lillian B., Silva, Israel T., Oliveira, Thiago Y., Rosales, Rafael A., Parrish, Erica H., Learn, Gerald H., Hahn, Beatrice H., Czartoski, Julie L., McElrath, M. Juliana, Lehmann, Clara, Klein, Florian, Caskey, Marina, Walker, Bruce D., Siliciano, Janet D., Siliciano, Robert F., Jankovic, Mila, Nussenzweig, Michel C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29.01.2015
• Subjects: Alu Elements; antiretroviral agents; CD4-positive T-lymphocytes; CD4-Positive T-Lymphocytes - virology; Clone Cells; clones; controllers; Defective Viruses - genetics; Defective Viruses - physiology; genome; HIV Infections - drug therapy; HIV Infections - virology; HIV-1 - genetics; HIV-1 - physiology; Human immunodeficiency virus 1; Humans; Immunologic Memory; landscapes; proviruses; Proviruses - physiology; Single-Cell Analysis; therapeutics; Virus Integration; Virus Latency

The barrier to curing HIV-1 is thought to reside primarily in CD4+ T cells containing silent proviruses. To characterize these latently infected cells, we studied the integration profile of HIV-1 in viremic progressors, individuals receiving antiretroviral therapy, and viremic controllers. Clonally...