Zeolite molecular sieves have dramatic acid-base effects on enzymes in nonaqueous media

• Published in: Biotechnology and bioengineering Vol. 77; no. 3; pp. 296 - 305
• Main Authors: Fontes, Nuno, Partridge, Johann, Halling, Peter J., Barreiros, Susana
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 05.02.2002; Wiley
• Subjects: Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Buffers; Crosslinking; Enzyme kinetics; enzymes; Enzymes - chemistry; Fundamental and applied biological sciences. Psychology; Hydration; Hydrogen-Ion Concentration; Ion exchange; ionization; Methods. Procedures. Technologies; Molecular sieves; pH effects; Reaction kinetics; Solvents - chemistry; Subtilisin - chemistry; supercritical fluids; water activity; zeolites; Zeolites - chemistry; Peptidases; Serine endopeptidases; Water activity; Enzyme; Nonaqueous media; Supercritical solvent; Hydrolases; Enzymatic catalysis; Subtilisin; Zeolite

Zeolite molecular sieves very commonly are used as in situ drying agents in reaction mixtures of enzymes in nonaqueous media. They often affect enzyme behavior, and this has been interpreted in terms of altered hydration. Here, we show that zeolites can also have dramatic acid–base effects on enzyme...