Comparative evaluation of vacuum-based surface sampling methods for collection of Bacillus spores
In this study, four commonly-used sampling devices (vacuum socks, 37mm 0.8μm mixed cellulose ester (MCE) filter cassettes, 37mm 0.3μm polytetrafluoroethylene (PTFE) filter cassettes, and 3M™ forensic filters) were comparatively evaluated for their ability to recover surface-associated spores. Aeroso...
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Published in | Journal of microbiological methods Vol. 95; no. 3; pp. 389 - 396 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.12.2013
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Subjects | |
Online Access | Get full text |
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Summary: | In this study, four commonly-used sampling devices (vacuum socks, 37mm 0.8μm mixed cellulose ester (MCE) filter cassettes, 37mm 0.3μm polytetrafluoroethylene (PTFE) filter cassettes, and 3M™ forensic filters) were comparatively evaluated for their ability to recover surface-associated spores. Aerosolized spores (~105CFUcm−2) of a Bacillus anthracis surrogate were allowed to settle onto three material types (concrete, carpet, and upholstery). Ten replicate samples were collected using each vacuum method, from each material type. Stainless steel surfaces, inoculated simultaneously with test materials, were sampled with pre-moistened wipes. Wipe recoveries were utilized to normalize vacuum-based recoveries across trials. Recovery (CFUcm−2) and relative recovery (vacuum recovery/wipe recovery) were determined for each method and material type. Recoveries and relative recoveries ranged from 3.8×103 to 7.4×104CFUcm−2 and 0.035 to 1.242, respectively. ANOVA results indicated that the 37mm MCE method exhibited higher relative recoveries than the other methods when used for sampling concrete or upholstery. While the vacuum sock resulted in the highest relative recoveries on carpet, no statistically significant difference was detected. The results of this study may be used to guide selection of sampling approaches following biological contamination incidents.
•Four vacuum-based methods were evaluated for the collection of spores from surfaces.•On concrete and upholstery, the 37mm MCE device had highest recoveries.•For carpet, the 37mm MCE and vacuum sock devices showed highest recoveries.•Laboratory processing times were shortest for the vacuum sock samples.•Sample collection times were greatest for the 37mm cassette devices. |
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Bibliography: | http://dx.doi.org/10.1016/j.mimet.2013.10.015 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Undefined-2 |
ISSN: | 0167-7012 1872-8359 |
DOI: | 10.1016/j.mimet.2013.10.015 |