Succinate Coenzyme A Ligase Beta-Like Protein from Trichinella spiralis Suppresses the Immune Functions of Rat PBMCs in Vitro and Inhibits the Secretions of Interleukin-17 in Vivo

• Published in: Vaccines (Basel) Vol. 7; no. 4; p. 167
• Main Authors: Sun, Xiaoke, Li, Yin, Naqvi, Muhammad Ali-Ul-Husnain, Naqvi, Sana Zahra, Chu, Wen, Xu, Lixin, Song, Xiaokai, Li, Xiangrui, Yan, Ruofeng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.11.2019; MDPI
• Subjects: Apoptosis; Autoimmune diseases; Biotechnology; Cell proliferation; Cloning; Coenzyme A; Cytokines; Disease prevention; Flow cytometry; Graft rejection; il17; Immune response; Immune system; Immunofluorescence; Immunoglobulin G; Immunomodulation; immunomodulatory; In vivo methods and tests; Incubation; Infections; Inflammatory diseases; Interleukin 17; Interleukin 4; Interleukin 9; Kinases; Laboratory animals; Larvae; Leukocyte migration; Leukocytes (mononuclear); Microorganisms; Mitochondrial DNA; Monocytes; Muscles; Musculoskeletal system; Nematodes; Nitric oxide; Parasites; Pathogenesis; Peripheral blood mononuclear cells; Phagocytosis; Proteins; Secretions; Substrates; sucla-β; Synergism; Trichinella spiralis; trichinella spiralis: rat pbmcs; Vaccines; γ-Interferon; China; cytokines; Trichinella spiralis: rat PBMCs; IL17; SUCLA-β; immunomodulatory
• ISSN: 2076-393X; 2076-393X
• DOI: 10.3390/vaccines7040167

Succinate Coenzyme A ligase beta-like protein (SUCLA-β) is a subunit of Succinyl-coenzyme A synthetase, which is involved in substrate synergism, unusual kinetic reaction in which the presence of SUCLA-β for one partial reaction stimulates another partial reaction. is a parasitic nematode, which may hinder the development of autoimmune diseases. Immunomodulatory effects of SUCLA-β from in the parasite-host interaction are unidentified. In this study the gene encoding SUCLA-β was cloned and expressed. Binding activities of recombinant SUCLA-β (rTs-SUCLA-β) to rat peripheral blood mononuclear cells (PBMCs) were checked by immunofluorescence assay (IFA) and the immuno-regulatory effects of rTs-SUCLA-β on cell migration, cell proliferation, nitric oxide (NO) production and apoptosis were observed by co-incubation of rTs-SUCLA-β with rat PBMCs in vitro, while cytokine secretions in rTs-SUCLA-β treated rats were evaluated in vivo. Furthermore, phagocytosis of monocytes was detected by flow cytometry and effects of rTs-SUCLA-β-induced protective immunity on adult worms and muscle larva were evaluated in rats. The IFA results revealed that rTs-SUCLA-β could bind to rat PBMCs. Treatment of PBMCs with rTs-SUCLA-β significantly decreased the monocyte phagocytosis, cell migration and cell proliferation, while NO production and apoptosis of PBMCs were unaffected. Results of the in vivo study showed that the IL-17 secretion decreased significantly after rTs-SUCLA-β administration in rats, while no significant effects were observed on the secretions of IFN-γ, IL-9, TGF-β and IL-4. Moreover, significant reduction of muscle larvae burden and significant increase in anti-rTs-SUCLA-β immunoglobulin level of IgG, IgG1 and IgG2a was observed in rTs-SUCLA-β-administered rats. The results indicated that rTs-SUCLA-β may be a potential target for controlling infection by suppressing the immune functions of the rat PBMCs and by reducing the parasite burden. Additionally it may also contribute to the treatment of autoimmune diseases and graft rejection by suppressing IL-17 immune response in the host.