Hepatitis B Virus DNA-negative Dane Particles Lack Core Protein but Contain a 22-kDa Precore Protein without C-terminal Arginine-rich Domain

• Published in: The Journal of biological chemistry Vol. 280; no. 23; pp. 21713 - 21719
• Main Authors: Kimura, Tatsuji, Ohno, Nobuhiko, Terada, Nobuo, Rokuhara, Akinori, Matsumoto, Akihiro, Yagi, Shintaro, Tanaka, Eiji, Kiyosawa, Kendo, Ohno, Shinichi, Maki, Noboru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.06.2005; American Society for Biochemistry and Molecular Biology
• Subjects: Antibodies, Monoclonal - chemistry; Antigens, Viral - chemistry; Arginine - chemistry; Blotting, Western; Capsid; Centrifugation, Density Gradient; Chromatography, Gel; Cytoplasm - metabolism; DNA, Viral - metabolism; Dose-Response Relationship, Drug; Female; Genome, Viral; Hepatitis B - blood; Hepatitis B - virology; Hepatitis B virus; Hepatitis B virus - genetics; Humans; Immunoenzyme Techniques; Immunoprecipitation; Male; Mass Spectrometry; Microscopy, Electron; Models, Genetic; Protein Structure, Tertiary; Protein Transport; Recombinant Proteins - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sucrose - pharmacology; Viral Core Proteins - chemistry

DNA-negative Dane particles have been observed in hepatitis B virus (HBV)-infected sera. The capsids of the empty particles are thought to be composed of core protein but have not been studied in detail. In the present study, the protein composition of the particles was examined using new enzyme imm...