Structural Characterization and Immunochemical Detection of a Fluorophore Derived from 4-Hydroxy-2-Nonenal and Lysine

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 95; no. 14; pp. 7975 - 7980
• Main Authors: Tsai, Lin, Szweda, Pamela A., Vinogradova, Olga, Szweda, Luke I.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 07.07.1998; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: Adducts; Aldehydes - chemistry; Aldehydes - immunology; Animals; Antibodies; Atoms; Biochemistry; Biological Sciences; Cellular biology; Cross-Linking Reagents; Enzyme linked immunosorbent assay; Female; Fluorescence; Fluorescent Dyes - chemistry; Gels; Immunization; Immunoassay - methods; Lipids; Lysine - chemistry; Lysine - immunology; Polyclonal antibodies; Rabbits

Aging and the progression of certain degenerative diseases are accompanied by increases in intracellular fluorescent material, termed ``lipofuscin'' and ceroid, respectively. These pigments are observed within granules composed, in part, of damaged protein and lipid. Modification of variou...