Choices on sampling, sequencing, and analyzing DNA influence the estimation of community composition of plant fungal symbionts

• Published in: Applications in plant sciences Vol. 9; no. 9-10; pp. e11449 - n/a
• Main Authors: Cale, Jonathan A., Scott, Natalie, Pec, Gregory J., Landhäusser, Simon M., Karst, Justine
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.09.2021; John Wiley and Sons Inc; Wiley
• Subjects: Bioinformatics; boreal forest; Carbon; Community composition; Community ecology; Deoxyribonucleic acid; DNA; DNA sequencing; ectomycorrhizal fungi; Ectomycorrhizas; Fungi; Illumina; jack pine; Nucleotide sequence; Nutrition; Plant communities; Plant sciences; Review; Roots; Sanger sequencing; Soil microorganisms; soils; Symbionts; Taxonomy; Thermal cycling; United States > US; boreal forest; Sanger sequencing; roots; soils; Illumina; ectomycorrhizal fungi; jack pine

Plant root symbionts, namely mycorrhizal fungi, can be characterized using a variety of methods, but most of these rely on DNA. While Sanger sequencing still fulfills particular research objectives, next‐generation sequencing currently dominates the field, thus understanding how the two methods diff...