N‐acetyl‐l‐cysteine Supplementation Improves Boar Spermatozoa Characteristics and Subsequent Fertilization and Embryonic Development
The effects of 1.0 mmN‐acetyl‐l‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen–thawed and preserved boar sperm were supplemented with 1.0 mm NAC and incubated for 60 min to allow capacitation to...
Saved in:
| Published in | Reproduction in domestic animals Vol. 47; no. 2; pp. 263 - 268 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.04.2012
Blackwell |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | The effects of 1.0 mmN‐acetyl‐l‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen–thawed and preserved boar sperm were supplemented with 1.0 mm NAC and incubated for 60 min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells–Awa staining technique. DNA damage was detected using single‐cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen–thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) more DNA damage (59.8 ± 1.0) compared to preserved sperm (32.0 ± 1.0%). Supplementing 1.0 mm NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) less DNA (39.2 ± 1.0%) damage compared to no antioxidant supplementation (52.7 ± 1.0%). Frozen–thawed sperm produced a significantly higher (p < 0.05) concentration of MDA (2.08 ± 0.05 μm MDA/107 cells) compared to preserved sperm (1.82 ± 0.05 μm MDA/107 cells), and non‐supplemented sperm produced a significantly higher (p < 0.05) concentration of MDA (3.62 ± 0.05 μm MDA/107 cells) compared to the 1.0 mm NAC‐supplemented sperm (0.28 ± 0.05 μm MDA/107 cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0 mm NAC improved the ability to use frozen–thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. |
|---|---|
| AbstractList | Contents
The effects of 1.0 mmN‐acetyl‐l‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen–thawed and preserved boar sperm were supplemented with 1.0 mm NAC and incubated for 60 min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells–Awa staining technique. DNA damage was detected using single‐cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen–thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) more DNA damage (59.8 ± 1.0) compared to preserved sperm (32.0 ± 1.0%). Supplementing 1.0 mm NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) less DNA (39.2 ± 1.0%) damage compared to no antioxidant supplementation (52.7 ± 1.0%). Frozen–thawed sperm produced a significantly higher (p < 0.05) concentration of MDA (2.08 ± 0.05 μm MDA/107 cells) compared to preserved sperm (1.82 ± 0.05 μm MDA/107 cells), and non‐supplemented sperm produced a significantly higher (p < 0.05) concentration of MDA (3.62 ± 0.05 μm MDA/107 cells) compared to the 1.0 mm NAC‐supplemented sperm (0.28 ± 0.05 μm MDA/107 cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0 mm NAC improved the ability to use frozen–thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. The effects of 1.0 mmN‐acetyl‐l‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen–thawed and preserved boar sperm were supplemented with 1.0 mm NAC and incubated for 60 min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells–Awa staining technique. DNA damage was detected using single‐cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen–thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) more DNA damage (59.8 ± 1.0) compared to preserved sperm (32.0 ± 1.0%). Supplementing 1.0 mm NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) less DNA (39.2 ± 1.0%) damage compared to no antioxidant supplementation (52.7 ± 1.0%). Frozen–thawed sperm produced a significantly higher (p < 0.05) concentration of MDA (2.08 ± 0.05 μm MDA/107 cells) compared to preserved sperm (1.82 ± 0.05 μm MDA/107 cells), and non‐supplemented sperm produced a significantly higher (p < 0.05) concentration of MDA (3.62 ± 0.05 μm MDA/107 cells) compared to the 1.0 mm NAC‐supplemented sperm (0.28 ± 0.05 μm MDA/107 cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0 mm NAC improved the ability to use frozen–thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. Contents The effects of 1.0 m m N ‐acetyl‐ l ‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen–thawed and preserved boar sperm were supplemented with 1.0 m m NAC and incubated for 60 min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells–Awa staining technique. DNA damage was detected using single‐cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen–thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) more DNA damage (59.8 ± 1.0) compared to preserved sperm (32.0 ± 1.0%). Supplementing 1.0 m m NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) less DNA (39.2 ± 1.0%) damage compared to no antioxidant supplementation (52.7 ± 1.0%). Frozen–thawed sperm produced a significantly higher (p < 0.05) concentration of MDA (2.08 ± 0.05 μ m MDA/10 7 cells) compared to preserved sperm (1.82 ± 0.05 μ m MDA/10 7 cells), and non‐supplemented sperm produced a significantly higher (p < 0.05) concentration of MDA (3.62 ± 0.05 μ m MDA/10 7 cells) compared to the 1.0 m m NAC‐supplemented sperm (0.28 ± 0.05 μ m MDA/10 7 cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0 m m NAC improved the ability to use frozen–thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. The effects of 1.0 mmN-acetyl-l-cysteine (NAC) supplementation during the incubation of frozen-thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen-thawed and preserved boar sperm were supplemented with 1.0 mm NAC and incubated for 60 min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells-Awa staining technique. DNA damage was detected using single-cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen-thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) more DNA damage (59.8 ± 1.0) compared to preserved sperm (32.0 ± 1.0%). Supplementing 1.0 mm NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) less DNA (39.2 ± 1.0%) damage compared to no antioxidant supplementation (52.7 ± 1.0%). Frozen-thawed sperm produced a significantly higher (p < 0.05) concentration of MDA (2.08 ± 0.05 μm MDA/10(7) cells) compared to preserved sperm (1.82 ± 0.05 μm MDA/10(7) cells), and non-supplemented sperm produced a significantly higher (p < 0.05) concentration of MDA (3.62 ± 0.05 μm MDA/10(7) cells) compared to the 1.0 mm NAC-supplemented sperm (0.28 ± 0.05 μm MDA/10(7) cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0 mm NAC improved the ability to use frozen-thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. Contents The effects of 1.0mmN-acetyl-l-cysteine (NAC) supplementation during the incubation of frozen-thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen-thawed and preserved boar sperm were supplemented with 1.0mm NAC and incubated for 60min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells-Awa staining technique. DNA damage was detected using single-cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen-thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p<0.05) more DNA damage (59.8±1.0) compared to preserved sperm (32.0±1.0%). Supplementing 1.0mm NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p<0.05) less DNA (39.2±1.0%) damage compared to no antioxidant supplementation (52.7±1.0%). Frozen-thawed sperm produced a significantly higher (p<0.05) concentration of MDA (2.08±0.05μm MDA/107cells) compared to preserved sperm (1.82±0.05μm MDA/107cells), and non-supplemented sperm produced a significantly higher (p<0.05) concentration of MDA (3.62±0.05μm MDA/107 cells) compared to the 1.0mm NAC-supplemented sperm (0.28±0.05μm MDA/107cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0mm NAC improved the ability to use frozen-thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. [PUBLICATION ABSTRACT] The effects of 1.0 mmN-acetyl-l-cysteine (NAC) supplementation during the incubation of frozen-thawed and preserved boar sperm were studied in addition to subsequent oocyte IVF. Frozen-thawed and preserved boar sperm were supplemented with 1.0 mm NAC and incubated for 60 min to allow capacitation to occur followed by the addition of calcium ionophore 23187 to induce the acrosome reaction. The number of sperm having undergone the acrosome reaction was determined using the Wells-Awa staining technique. DNA damage was detected using single-cell gel electrophoresis. Membrane lipid peroxidation was estimated by the end point generation of malondialdehyde (MDA). Frozen-thawed sperm was not different in the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) more DNA damage (59.8 ± 1.0) compared to preserved sperm (32.0 ± 1.0%). Supplementing 1.0 mm NAC did not have an effect on the ability of sperm to undergo the acrosome reaction but did have significantly (p < 0.05) less DNA (39.2 ± 1.0%) damage compared to no antioxidant supplementation (52.7 ± 1.0%). Frozen-thawed sperm produced a significantly higher (p < 0.05) concentration of MDA (2.08 ± 0.05 μm MDA/10(7) cells) compared to preserved sperm (1.82 ± 0.05 μm MDA/10(7) cells), and non-supplemented sperm produced a significantly higher (p < 0.05) concentration of MDA (3.62 ± 0.05 μm MDA/10(7) cells) compared to the 1.0 mm NAC-supplemented sperm (0.28 ± 0.05 μm MDA/10(7) cells. Supplementation or semen storage method had no effect on IVF or embryonic development. These results indicate that supplementation with 1.0 mm NAC improved the ability to use frozen-thawed boar sperm during IVF as it reduces the DNA fragmentation and lipid peroxidation of the sperm. |
| Author | Casey, SJ Whitaker, BD Taupier, R |
| Author_xml | – sequence: 1 fullname: Whitaker, BD – sequence: 2 fullname: Casey, SJ – sequence: 3 fullname: Taupier, R |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25589590$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/21762214$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkk9v0zAYxiM0xP7AV4AICXFK8X87Bw6jW8fQNCrKGDfLcd9CuiQOdjranThz4jPySXBIKRInLFl-Jf-ex6_9-DDZa1wDSZJiNMJxvFiOMKN5hjjFI4IwHiGsmBqt7yUHu4295ADlVGRCCrWfHIawRAhzJeWDZJ9gKQjB7CD5fvnz2w9jodtUsein3YQOygbS2aptK6ih6UxXuiY9r1vvbiGkr5zx6awFX5vO3TmTjj8bb2wHvgxdaUNqmnlUFwG-rKI6nYDvyqq8G2z6zdO68BvXlDY9gVuoXNuf8jC5vzBVgEfb9Si5mpy-H7_OLt6enY-PLzLLOVUZECAsJwwJSoTFSCk-RwRJKoBQg1TBZIHpPEcFJdLOJcmNwAXiueCiYIrRo-T54BuvExsMna7LYKGqTANuFXROVI5kjmkkn_5DLt3KN7G5HkIMY4YipAbIeheCh4VufVkbv9EY6T4tvdR9KLoPRfdp6d9p6XWUPt76r4oa5jvhn3gi8GwLmGBNtfCmsWX4y3Gucp73PbwcuK9lBZv_bkC_Oznuq6jPBn0MENY7vfE3Wkgqub6-PNPTN5Prj1M21R8i_2TgF8Zp8ynmrq9m0Zmh-Mcwj0_3C2xNzfg |
| CitedBy_id | crossref_primary_10_1016_j_theriogenology_2023_12_030 crossref_primary_10_30607_kvj_435112 crossref_primary_10_1016_j_theriogenology_2015_07_017 crossref_primary_10_1016_j_theriogenology_2021_07_023 crossref_primary_10_3390_ani12182431 crossref_primary_10_1016_j_repbio_2020_06_002 crossref_primary_10_3390_ani13193040 crossref_primary_10_1016_j_taap_2015_09_010 crossref_primary_10_1016_j_smallrumres_2023_107166 crossref_primary_10_1111_and_12293 crossref_primary_10_1016_j_cryobiol_2013_06_002 crossref_primary_10_1071_AN16274 crossref_primary_10_5938_youton_50_157 crossref_primary_10_1016_j_anireprosci_2024_107496 crossref_primary_10_1262_jrd_2014_055 crossref_primary_10_3390_ani13182829 |
| Cites_doi | 10.1016/S0093-691X(01)00702-6 10.1016/S0093-691X(98)00180-0 10.1016/S0891-5849(98)00275-5 10.1016/j.theriogenology.2008.02.010 10.1016/S0093-691X(02)01125-1 10.1095/biolreprod60.6.1536 10.1002/bmc.583 10.1111/j.1439-0531.2005.00626.x 10.1071/RD08037 10.1095/biolreprod61.3.828 10.1095/biolreprod59.5.1037 10.1016/S0378-4320(03)00049-6 10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7 10.1016/j.rvsc.2010.03.025 10.1016/S0093-691X(01)00670-7 10.1016/j.reprotox.2007.04.004 10.1016/j.theriogenology.2007.05.001 10.1177/002215540104900912 10.1016/S0378-4320(00)00157-3 10.1002/(SICI)1098-2795(199911)54:3<292::AID-MRD10>3.0.CO;2-F 10.4067/S0716-97602006000500006 10.1093/toxsci/kfi300 10.1095/biolreprod40.3.525 10.1111/j.1439-0272.2009.00964.x 10.1007/s00418-004-0677-x 10.1038/aja.2009.78 10.1016/S0093-691X(97)00270-7 10.2164/jandrol.107.002725 10.1016/0891-5849(89)90066-X 10.1016/j.cryobiol.2008.12.004 10.1002/mrd.1052 |
| ContentType | Journal Article |
| Copyright | 2011 Blackwell Verlag GmbH 2015 INIST-CNRS 2011 Blackwell Verlag GmbH. |
| Copyright_xml | – notice: 2011 Blackwell Verlag GmbH – notice: 2015 INIST-CNRS – notice: 2011 Blackwell Verlag GmbH. |
| DBID | FBQ BSCLL IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QO 8FD FR3 P64 7X8 |
| DOI | 10.1111/j.1439-0531.2011.01848.x |
| DatabaseName | AGRIS Istex Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Biotechnology Research Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Engineering Research Database Biotechnology Research Abstracts Technology Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE Engineering Research Database MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Veterinary Medicine Agriculture |
| EISSN | 1439-0531 |
| EndPage | 268 |
| ExternalDocumentID | 2610102431 10_1111_j_1439_0531_2011_01848_x 21762214 25589590 RDA1848 ark_67375_WNG_PJFWXP4P_V US201400011513 |
| Genre | article Research Support, Non-U.S. Gov't Journal Article Feature |
| GroupedDBID | --- .3N .GA .Y3 05W 0R~ 10A 123 1OB 1OC 29P 31~ 33P 36B 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5HH 5LA 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHHS AAHTB AAJUZ AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABCVL ABEML ABHUG ABJNI ABPEJ ABPTK ABPVW ABWRO ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACGFS ACGOD ACIWK ACPOU ACPRK ACSCC ACXBN ACXME ACXQS ADAWD ADBBV ADDAD ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN AEEZP AEGXH AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFEBI AFFPM AFGKR AFPWT AFRAH AFVGU AFZJQ AGJLS AHEFC AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BY8 C45 CAG COF CS3 D-E D-F DCZOG DPXWK DR2 DRFUL DRSTM DU5 EBS ECGQY EJD ESX EYRJQ F00 F01 F04 F5P FBQ FEDTE FZ0 G-S G.N GODZA H.T H.X HF~ HVGLF HVLQZ HZI HZ~ IHE IX1 J0M K48 LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ O66 O9- OVD P2W P2X P4D PALCI PQQKQ Q.N Q11 QB0 R.K RIWAO RJQFR ROL RX1 SAMSI SUPJJ TEORI UB1 V8K W8V W99 WBKPD WIH WIK WOHZO WOIKV WPGGZ WQJ WRC WXSBR XG1 ZZTAW ~IA ~KM ~WT AHBTC BSCLL AAHBH AITYG HGLYW OIG AAPBV IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QO 8FD FR3 P64 7X8 |
| ID | FETCH-LOGICAL-c5538-e2e2492406326c10885d020736e23a08b47b13d90b327cd729a61b059656b4843 |
| IEDL.DBID | DR2 |
| ISSN | 0936-6768 |
| IngestDate | Sat Aug 17 02:50:28 EDT 2024 Fri Aug 30 23:02:51 EDT 2024 Fri Aug 23 00:54:12 EDT 2024 Sat Sep 28 07:49:09 EDT 2024 Sun Oct 22 16:07:16 EDT 2023 Sat Aug 24 00:57:27 EDT 2024 Wed Jan 17 05:02:35 EST 2024 Wed Dec 27 19:27:28 EST 2023 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | Embryonic development Vertebrata Spermatozoa Mammalia Fertilization Characteristic Supplementation Germinal cell |
| Language | English |
| License | CC BY 4.0 2011 Blackwell Verlag GmbH. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c5538-e2e2492406326c10885d020736e23a08b47b13d90b327cd729a61b059656b4843 |
| Notes | http://dx.doi.org/10.1111/j.1439-0531.2011.01848.x istex:9AC65746AF77D3EE00301E718065AB7A8E4EA8E3 ArticleID:RDA1848 ark:/67375/WNG-PJFWXP4P-V ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 21762214 |
| PQID | 928041140 |
| PQPubID | 26873 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_928907913 proquest_journals_928041140 crossref_primary_10_1111_j_1439_0531_2011_01848_x pubmed_primary_21762214 pascalfrancis_primary_25589590 wiley_primary_10_1111_j_1439_0531_2011_01848_x_RDA1848 istex_primary_ark_67375_WNG_PJFWXP4P_V fao_agris_US201400011513 |
| PublicationCentury | 2000 |
| PublicationDate | April 2012 |
| PublicationDateYYYYMMDD | 2012-04-01 |
| PublicationDate_xml | – month: 04 year: 2012 text: April 2012 |
| PublicationDecade | 2010 |
| PublicationPlace | Oxford, UK |
| PublicationPlace_xml | – name: Oxford, UK – name: Berlin – name: Germany – name: Oxford |
| PublicationTitle | Reproduction in domestic animals |
| PublicationTitleAlternate | Reprod Domest Anim |
| PublicationYear | 2012 |
| Publisher | Blackwell Publishing Ltd Blackwell |
| Publisher_xml | – name: Blackwell Publishing Ltd – name: Blackwell |
| References | Johnson LA, Weitze KF, Fiser P, Maxwell WMC, 2000: Storage of boar semen. Anim Reprod Sci 62, 143-172. Ali AA, Bilodeau JF, Sirard MA, 2003: Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development. Theriogenology 59, 939-949. Chatterjee S, Gagnon C, 2001: Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev 59, 451-458. Nishikimi A, Mukai J, Yamada M, 1999: Nuclear translocation of nuclear factor kappa B in early 1-cell mouse embryos. Biol Reprod 60, 1536-1541. Abeydeera LR, Wang WH, Cantley TC, Prather RS, Day BN, 1998: Presence of mercaptoethanol can increase the glutathione content of pig oocytes matured in vitro and the rate of blastocyst development after in vitro fertilization. Theriogenology 50, 747-756. Abeydeera LR, 2002: In vitro production of embryos in swine. Theriogenology 57, 257-273. Tardiff S, Sirard MA, Sullivan R, Bailey J, 1999: Identification of capacitation-associated phosphoproteins in porcine sperm electroportated with ATP-γ-32 P. Mol Reprod Dev 54, 292-302. Whitaker BD, Carle B, Mukai T, Simpson A, Vu L, Knight JW, 2008: Effect of exogenous glutathione supplementation on motility, viability, and DNA integrity of frozen-thawed boar semen. Anim Reprod 5, 127-131. du Plessis SS, Hagenaar K, Lampiao F, 2010: The in vitro effects of melatonin on human sperm function and its scavenging activities on NO and ROS. Andrologia 42, 112-116. Dapino DG, Marini PE, Cabada MO, 2006: Effect of heparin on in vitro capacitation of boar sperm. Biol Res 39, 631-639. Whitaker BD, Knight JW, 2009: Effects of N-acetyl-cysteine and N-acetyl-cysteine-amide supplementation on in vitro matured porcine oocytes. Reprod Dom Anim 45, 755-759. Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Kumar BM, Balasubramanian S, Rho GJ, 2009: Effect of alpha-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology 58, 181-189. Ornoy A, 2007: Embryonic oxidative stress as a mechanism of teratogenesis with special emphasis on diabetic embryopathy. Reprod Toxicol 24, 31-41. Fraser L, Strzeżek J, 2004: The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C. Folia Histochem Cytobiol 40, 530-536. Yamauchi N, Nagai T, 1999: Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol Reprod 61, 828-833. Abeydeera LR, Day BN, 1997: In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium. Theriogenology, 48, 537-544. Aitken JR, Gordon E, Harkiss D, Twigg JP, Milne P, Zoe J, Irvine DS, 1998: Relative impact of oxidation stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod 59, 1037-1046. Armstrong JS, Rajasekaran M, Chamulitrat W, Gatti P, Hellstrom WJ, Sikka SC, 1999: Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radic Biol Med 26, 869-880. Wu W, Goldstein G, Adams C, Matthews RH, Ercal N, 2006: Separation and quantification of N-acetyl-L-cysteine and N-acetyl-cysteine-amide by HPLC with fluorescence detection. Biomed Chromatogr 20, 415-422. Whitaker BD, Knight JW, 2008: Mechanisms of oxidative stress in porcine oocytes and the role of antioxidants. Reprod Fertil Dev 20, 694-702. Coyan K, Başpınar N, Bucak MN, Akalın PP, Ataman MB, Omür AD, Güngör S, Küçükgünay S, Ozkalp B, Sarıözkan S, 2010: Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen. Res Vet Sci 89, 426-431. Myatt L, Cui X, 2004: Oxidative stress in the placenta. Histochem Cell Biol 122, 369-382. Aruoma OI, Halliwell B, Hoey BM, Butler J, 1989: The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med 6, 593-597. Berger T, Turner K, Meizel S, Hendrick J, 1989: Zona pellucida-induced acrosome reaction in boar sperm. Biol Reprod 40, 525-530. Fraser L, Strzeżek J, 2005: Effects of freezing-thawing on DNA integrity of boar spermatozoa assessed by neutral comet assay. Reprod Domest Anim 40, 530-536. Li SF, Liu HX, Zhang YB, Yan YC, Li YP, 2010: The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro. Asian J Androl 12, 247-256. Hernandez M, Roca J, Calvete J, Sanz L, Muino-Blanco T, Cebrian-Perez JA, Valazquez JM, Martinez EA, 2007: Cryosurvival and in vitro fertilizing capacity postthaw is improved when boar spermatozoa are frozen in the presence of seminal plasma from good freezer boars. J Androl 28, 689-697. Bilodeau JF, Blanchette S, Cormier N, Sirard MA, 2002: Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology 57, 1105-1122. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C, 2000: Levels of antioxidant defences are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev 55, 282-288. Peña FJ, Johannisson A, Wallgren M, Rodrıguez-Martınez H, 2003: Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim Reprod Sci 78, 85-98. Moran JM, Madejón L, Ortega-Ferrusola C, Peña FJ, 2008: Nitric oxide induces caspase activity in boar spermatozoa. Theriogenology 70, 91-96. Xu D, Chen Y, Wang H, Zhao L, Wang J, Wei W, 2005: Effect of N-acetylcysteine on lipopolysaccharide-induced intrauterine fetal death and intra-uterine growth retardation in mice. Toxicol Sci 88, 525-533. Petters RM, Wells CD, 1993: Culture of pig embryos. J Reprod Fertil Suppl 48, 61-73. Fraser L, Strzeżek J, 2007: Is there a relationship between the chromatin status and DNA fragmentation of boar spermatozoa following freezing-thawing? Theriogenology 68, 248-257. Nadin SB, Vargas-Roig LM, Ciocca DR, 2001: A silver staining method for single-cell gel assay. J Histochem Cytochem 49, 1183-1186. 1989; 40 2010; 12 1993; 48 2009; 45 2004; 122 2004; 40 1997; 48 2002; 57 1989; 6 2006; 39 1999; 26 2005; 40 2003; 59 2008; 5 2001; 49 1999; 61 1999; 60 2008; 70 2005; 88 2003; 78 2010; 89 2007; 28 1998; 59 2009; 58 2010; 42 2006; 20 2000; 55 2000; 62 1999; 54 2001; 59 1998; 50 2008; 20 2007; 68 2007; 24 e_1_2_8_29_1 e_1_2_8_24_1 e_1_2_8_25_1 e_1_2_8_26_1 e_1_2_8_27_1 Whitaker BD (e_1_2_8_32_1) 2009; 45 Fraser L (e_1_2_8_15_1) 2004; 40 e_1_2_8_3_1 e_1_2_8_2_1 e_1_2_8_5_1 e_1_2_8_4_1 e_1_2_8_7_1 e_1_2_8_6_1 e_1_2_8_9_1 e_1_2_8_8_1 e_1_2_8_20_1 e_1_2_8_21_1 e_1_2_8_22_1 e_1_2_8_23_1 e_1_2_8_17_1 e_1_2_8_18_1 e_1_2_8_19_1 e_1_2_8_13_1 e_1_2_8_36_1 e_1_2_8_14_1 e_1_2_8_35_1 e_1_2_8_16_1 Whitaker BD (e_1_2_8_33_1) 2008; 5 e_1_2_8_10_1 e_1_2_8_31_1 e_1_2_8_11_1 e_1_2_8_34_1 e_1_2_8_12_1 Petters RM (e_1_2_8_28_1) 1993; 48 e_1_2_8_30_1 |
| References_xml | – volume: 58 start-page: 181 year: 2009 end-page: 189 article-title: Effect of alpha‐tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes publication-title: Cryobiology – volume: 45 start-page: 755 year: 2009 end-page: 759 article-title: Effects of N‐acetyl‐cysteine and N‐acetyl‐cysteine‐amide supplementation on matured porcine oocytes publication-title: Reprod Dom Anim – volume: 59 start-page: 1037 year: 1998 end-page: 1046 article-title: Relative impact of oxidation stress on the functional competence and genomic integrity of human spermatozoa publication-title: Biol Reprod – volume: 89 start-page: 426 year: 2010 end-page: 431 article-title: Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen publication-title: Res Vet Sci – volume: 28 start-page: 689 year: 2007 end-page: 697 article-title: Cryosurvival and fertilizing capacity postthaw is improved when boar spermatozoa are frozen in the presence of seminal plasma from good freezer boars publication-title: J Androl – volume: 24 start-page: 31 year: 2007 end-page: 41 article-title: Embryonic oxidative stress as a mechanism of teratogenesis with special emphasis on diabetic embryopathy publication-title: Reprod Toxicol – volume: 42 start-page: 112 year: 2010 end-page: 116 article-title: The effects of melatonin on human sperm function and its scavenging activities on NO and ROS publication-title: Andrologia – volume: 55 start-page: 282 year: 2000 end-page: 288 article-title: Levels of antioxidant defences are decreased in bovine spermatozoa after a cycle of freezing and thawing publication-title: Mol Reprod Dev – volume: 62 start-page: 143 year: 2000 end-page: 172 article-title: Storage of boar semen publication-title: Anim Reprod Sci – volume: 48 start-page: 537 year: 1997 end-page: 544 article-title: penetration of pig oocytes in a modified Tris‐buffered medium: effect of BSA, caffeine and calcium publication-title: Theriogenology – volume: 5 start-page: 127 year: 2008 end-page: 131 article-title: Effect of exogenous glutathione supplementation on motility, viability, and DNA integrity of frozen‐thawed boar semen publication-title: Anim Reprod – volume: 57 start-page: 257 year: 2002 end-page: 273 article-title: production of embryos in swine publication-title: Theriogenology – volume: 49 start-page: 1183 year: 2001 end-page: 1186 article-title: A silver staining method for single‐cell gel assay publication-title: J Histochem Cytochem – volume: 20 start-page: 415 year: 2006 end-page: 422 article-title: Separation and quantification of N‐acetyl‐L‐cysteine and N‐acetyl‐cysteine‐amide by HPLC with fluorescence detection publication-title: Biomed Chromatogr – volume: 122 start-page: 369 year: 2004 end-page: 382 article-title: Oxidative stress in the placenta publication-title: Histochem Cell Biol – volume: 50 start-page: 747 year: 1998 end-page: 756 article-title: Presence of mercaptoethanol can increase the glutathione content of pig oocytes matured and the rate of blastocyst development after fertilization publication-title: Theriogenology – volume: 70 start-page: 91 year: 2008 end-page: 96 article-title: Nitric oxide induces caspase activity in boar spermatozoa publication-title: Theriogenology – volume: 78 start-page: 85 year: 2003 end-page: 98 article-title: Antioxidant supplementation improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate publication-title: Anim Reprod Sci – volume: 48 start-page: 61 year: 1993 end-page: 73 article-title: Culture of pig embryos publication-title: J Reprod Fertil Suppl – volume: 68 start-page: 248 year: 2007 end-page: 257 article-title: Is there a relationship between the chromatin status and DNA fragmentation of boar spermatozoa following freezing‐thawing? publication-title: Theriogenology – volume: 61 start-page: 828 year: 1999 end-page: 833 article-title: Male pronuclear formation in denuded porcine oocytes after maturation in the presence of cysteamine publication-title: Biol Reprod – volume: 6 start-page: 593 year: 1989 end-page: 597 article-title: The antioxidant action of N‐acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid publication-title: Free Radic Biol Med – volume: 88 start-page: 525 year: 2005 end-page: 533 article-title: Effect of N‐acetylcysteine on lipopolysaccharide‐induced intrauterine fetal death and intra‐uterine growth retardation in mice publication-title: Toxicol Sci – volume: 40 start-page: 530 year: 2004 end-page: 536 article-title: The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C publication-title: Folia Histochem Cytobiol – volume: 40 start-page: 530 year: 2005 end-page: 536 article-title: Effects of freezing‐thawing on DNA integrity of boar spermatozoa assessed by neutral comet assay publication-title: Reprod Domest Anim – volume: 60 start-page: 1536 year: 1999 end-page: 1541 article-title: Nuclear translocation of nuclear factor kappa B in early 1‐cell mouse embryos publication-title: Biol Reprod – volume: 54 start-page: 292 year: 1999 end-page: 302 article-title: Identification of capacitation‐associated phosphoproteins in porcine sperm electroportated with ATP‐γ‐ P publication-title: Mol Reprod Dev – volume: 59 start-page: 451 year: 2001 end-page: 458 article-title: Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing publication-title: Mol Reprod Dev – volume: 40 start-page: 525 year: 1989 end-page: 530 article-title: Zona pellucida‐induced acrosome reaction in boar sperm publication-title: Biol Reprod – volume: 57 start-page: 1105 year: 2002 end-page: 1122 article-title: Reactive oxygen species‐mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase publication-title: Theriogenology – volume: 59 start-page: 939 year: 2003 end-page: 949 article-title: Antioxidant requirements for bovine oocytes varies during maturation, fertilization and development publication-title: Theriogenology – volume: 12 start-page: 247 year: 2010 end-page: 256 article-title: The protective effects of alpha‐ketoacids against oxidative stress on rat spermatozoa publication-title: Asian J Androl – volume: 20 start-page: 694 year: 2008 end-page: 702 article-title: Mechanisms of oxidative stress in porcine oocytes and the role of antioxidants publication-title: Reprod Fertil Dev – volume: 26 start-page: 869 year: 1999 end-page: 880 article-title: Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism publication-title: Free Radic Biol Med – volume: 39 start-page: 631 year: 2006 end-page: 639 article-title: Effect of heparin on capacitation of boar sperm publication-title: Biol Res – ident: e_1_2_8_11_1 doi: 10.1016/S0093-691X(01)00702-6 – ident: e_1_2_8_4_1 doi: 10.1016/S0093-691X(98)00180-0 – ident: e_1_2_8_7_1 doi: 10.1016/S0891-5849(98)00275-5 – ident: e_1_2_8_22_1 doi: 10.1016/j.theriogenology.2008.02.010 – volume: 45 start-page: 755 year: 2009 ident: e_1_2_8_32_1 article-title: Effects of N‐acetyl‐cysteine and N‐acetyl‐cysteine‐amide supplementation on in vitro matured porcine oocytes publication-title: Reprod Dom Anim contributor: fullname: Whitaker BD – ident: e_1_2_8_6_1 doi: 10.1016/S0093-691X(02)01125-1 – ident: e_1_2_8_25_1 doi: 10.1095/biolreprod60.6.1536 – ident: e_1_2_8_34_1 doi: 10.1002/bmc.583 – ident: e_1_2_8_16_1 doi: 10.1111/j.1439-0531.2005.00626.x – ident: e_1_2_8_31_1 doi: 10.1071/RD08037 – volume: 40 start-page: 530 year: 2004 ident: e_1_2_8_15_1 article-title: The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C publication-title: Folia Histochem Cytobiol contributor: fullname: Fraser L – ident: e_1_2_8_36_1 doi: 10.1095/biolreprod61.3.828 – ident: e_1_2_8_5_1 doi: 10.1095/biolreprod59.5.1037 – ident: e_1_2_8_27_1 doi: 10.1016/S0378-4320(03)00049-6 – ident: e_1_2_8_10_1 doi: 10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7 – ident: e_1_2_8_13_1 doi: 10.1016/j.rvsc.2010.03.025 – ident: e_1_2_8_2_1 doi: 10.1016/S0093-691X(01)00670-7 – ident: e_1_2_8_26_1 doi: 10.1016/j.reprotox.2007.04.004 – ident: e_1_2_8_17_1 doi: 10.1016/j.theriogenology.2007.05.001 – ident: e_1_2_8_24_1 doi: 10.1177/002215540104900912 – ident: e_1_2_8_20_1 doi: 10.1016/S0378-4320(00)00157-3 – ident: e_1_2_8_30_1 doi: 10.1002/(SICI)1098-2795(199911)54:3<292::AID-MRD10>3.0.CO;2-F – ident: e_1_2_8_14_1 doi: 10.4067/S0716-97602006000500006 – ident: e_1_2_8_35_1 doi: 10.1093/toxsci/kfi300 – ident: e_1_2_8_9_1 doi: 10.1095/biolreprod40.3.525 – ident: e_1_2_8_29_1 doi: 10.1111/j.1439-0272.2009.00964.x – volume: 5 start-page: 127 year: 2008 ident: e_1_2_8_33_1 article-title: Effect of exogenous glutathione supplementation on motility, viability, and DNA integrity of frozen‐thawed boar semen publication-title: Anim Reprod contributor: fullname: Whitaker BD – ident: e_1_2_8_23_1 doi: 10.1007/s00418-004-0677-x – ident: e_1_2_8_21_1 doi: 10.1038/aja.2009.78 – ident: e_1_2_8_3_1 doi: 10.1016/S0093-691X(97)00270-7 – ident: e_1_2_8_18_1 doi: 10.2164/jandrol.107.002725 – ident: e_1_2_8_8_1 doi: 10.1016/0891-5849(89)90066-X – ident: e_1_2_8_19_1 doi: 10.1016/j.cryobiol.2008.12.004 – volume: 48 start-page: 61 year: 1993 ident: e_1_2_8_28_1 article-title: Culture of pig embryos publication-title: J Reprod Fertil Suppl contributor: fullname: Petters RM – ident: e_1_2_8_12_1 doi: 10.1002/mrd.1052 |
| SSID | ssj0015877 |
| Score | 2.089075 |
| Snippet | The effects of 1.0 mmN‐acetyl‐l‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in addition to... Contents The effects of 1.0 mmN‐acetyl‐l‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in... The effects of 1.0 mmN-acetyl-l-cysteine (NAC) supplementation during the incubation of frozen-thawed and preserved boar sperm were studied in addition to... Contents The effects of 1.0 m m N ‐acetyl‐ l ‐cysteine (NAC) supplementation during the incubation of frozen–thawed and preserved boar sperm were studied in... Contents The effects of 1.0mmN-acetyl-l-cysteine (NAC) supplementation during the incubation of frozen-thawed and preserved boar sperm were studied in addition... |
| SourceID | proquest crossref pubmed pascalfrancis wiley istex fao |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 263 |
| SubjectTerms | Acetylcysteine - pharmacology acrosome reaction Acrosome Reaction - drug effects Animal reproduction Animals Biological and medical sciences boars calcium Cell Membrane - drug effects Cell Membrane - physiology Cryoprotective Agents - pharmacology Dietary supplements DNA DNA damage DNA fragmentation Embryo Culture Techniques - veterinary embryogenesis Embryos Female Fertilization in Vitro - veterinary Fundamental and applied biological sciences. Psychology gel electrophoresis Hogs Hormones in vitro fertilization Lipid Peroxidation Male Mammalian reproduction. General aspects Oocytes semen Semen Preservation - methods Semen Preservation - veterinary Sperm spermatozoa Spermatozoa - drug effects Swine Vertebrates: reproduction |
| Title | N‐acetyl‐l‐cysteine Supplementation Improves Boar Spermatozoa Characteristics and Subsequent Fertilization and Embryonic Development |
| URI | https://api.istex.fr/ark:/67375/WNG-PJFWXP4P-V/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1439-0531.2011.01848.x https://www.ncbi.nlm.nih.gov/pubmed/21762214 https://www.proquest.com/docview/928041140/abstract/ https://search.proquest.com/docview/928907913 |
| Volume | 47 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1NT9tAEB21nNpDP9IPXFq0B9SbI9u7_jqmlBQhEUXQQG6rtb3pIdRGtiMBJ86c-hv7SzqzdgxGHFDFIZKVjdfx6O3sW_vtG4CdDFmyJgeARay0LRR3bMSNsIUOQy6EQg5Pu5EPJ8H-TBzM_Xmrf6K9MI0_RPfAjUaGydc0wFVS3RvkPLYJRK0TJy5WoiHxSfLVI3501DlJuX5kijDi-j2wA6TYfVHPgx31ZqrnC1Ugf6XQX5B-UlUYwkVT--IhctrnumayGr-G5fo2G43Kcriqk2F6dc8B8mni8AZetZyWjRoQvoVnOh_Ay9GvsvX10AMYnJDsxuz9ZYft6_x3cDP5e_1Hpbq-PMMD-qRkLI1tzFQbbZTtBB3WPPzQFftWqJIdn9OEUhdXhWK7fctppvKMUTo0GvGajUk4ftbuNjWNe7-T8pIcgdkdxdR7mI33fu7u221xCDv1KUlrT5PZIfIRJKCpi8nSz5D6hjzQHldOlIgwcXkWOwn3wjTDNYQK3ISKDflBIiLBP8BGXuR6E5jPQ0xbXKQ8IoJLFoki0FRpK8xElCoL3DUQ5HnjASLvrp14LCn6kqIvTfTlhQWbiBipMNSVnB17tJA19NvlFnw1MOr6UuWS5HWhL08nP-T0YHw6n4qpPLFgu4ez7gRcBkaxHzsWbK2BJ9vUU8nYI0spvJwFrGvFnEEvglSui5X5SeyEMf2Vjw1cb7t2cXb0XGFBYED36BuWR99HdPTpf0_cghf4tdeooj7DRl2u9BckfHWybYbyP1DYRqg |
| link.rule.ids | 315,786,790,1382,27957,27958,46329,46753 |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9NAEB1BOQCHAuGjplD2gLg5ir3rr2MoDaE0UdQ2bW6rtb3h0NSuHEdqe-LMid_IL2Fm7Zi66gEhDpYsrb22R29n36xn3wC8T5Ela1IAmEdK20Lxno24EbbQQcCFUMjhaTfyaOwPp2J_5s3qckC0F6bSh2gW3GhkGH9NA5wWpG-Nch7ZhKJaihOjlbCLhPIBjn7PxFeHjZaU44WmDCNG8L7tI8lup_Xc2VNrrro_VzkyWDL-JWVQqiUacV5Vv7iLnrbZrpmuBk9gsf7QKkvlrLsq425yfUsD8j9Z4ils1rSW9SscPoN7OuvA4_63opb20B3onFDmjdn-y0b1H_3n8GP86_tPlejyaoEndCSkLY1tzBQcrZLbCT2sWv_QS_YxVwU7uqA5pcyvc8V226rTTGUpI49o0sRLNqDc8UW94dQ07p3HxRWJArMbSVMvYDrYO94d2nV9CDvxyE9rV5PeIVIS5KCJg_7SS5H9BtzXLle9MBZB7PA06sXcDZIUwwjlOzHVG_L8WISCv4SNLM_0FjCPB-i5uEh4SByXVBKFr6nYVpCKMFEWOGskyItKBkTeDJ94JMn6kqwvjfXlpQVbCBmp0NRLOT1yKZY1DNzhFnwwOGr6UsUZZdgFnjwdf5aT_cHpbCIm8sSCnRbQmhswEgwjL-pZsL1Gnqy9z1JGLqlK4eMsYE0rug36F6Qyna_MJVEviOhVXlV4_dO1gxOk6wgLfIO6v_5gefipT2ev__XGd_BweDw6kAdfxl-34RFe4lZJUm9goyxW-i3yvzLeMeP6N8I6Sso |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9NAEB1BkRAc-AiUmtKyB8TNke1dfx1DU7cUGkUtaXNbre0Nh5Q4Shyp7YkzJ34jv4SZtWPqqgeEOESytPbGHr2dfWO_fQvwLkeWrMkBYBIrbQvFHRtxI2yhw5ALoZDD02rk40FwOBJHY39c659oLUzlD9G8cKORYfI1DfB5Prk1yHlsE4hqJ04sVqIu8skHIuAeIbx_0lhJuX5kdmHEAj6wA-TYbVXPnT21pqr7E1UggaXYX5KAUi0xhpNq84u72Gmb7JrZKnkK0_VzViKVaXdVpt3s-pYF5P8JxDN4UpNa1qtQ-Bzu6VkHHve-LmpjD92BzhnpbsziX3Zcf89_AT8Gv77_VJkury7wgH4ZOUtjGzPbjVbSdsIOq95-6CX7UKgFO53TjFIW14Vie23PaaZmOaN8aETiJUtIOX5RLzc1jfvf0sUVWQKzG5KplzBK9r_sHdr17hB25lOW1p4mt0MkJMhAMxezpZ8j9w15oD2unCgVYeryPHZS7oVZjkWECtyUdhvyg1REgm_CxqyY6S1gPg8xb3GR8YgYLnkkikDTVlthLqJMWeCugSDnlQmIvFk88VhS9CVFX5roy0sLthAxUmGol3J06lEla_i3yy14b2DU9KUWU9LXhb48HxzI4VFyPh6KoTyzYLeFs-YCrAOj2I8dC7bXwJN17lnK2CNPKfw7C1jTikmDvgSpmS5W5pTYCWO6lVcVXP907eL06LnCgsCA7q8fWJ70e3T0-l8vfAsPh_1Efv44-LQNj_AMr1JIvYGNcrHSO0j-ynTXjOrfl1tJeQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=N%E2%80%90acetyl%E2%80%90l%E2%80%90cysteine+Supplementation+Improves+Boar+Spermatozoa+Characteristics+and+Subsequent+Fertilization+and+Embryonic+Development&rft.jtitle=Reproduction+in+domestic+animals&rft.au=Whitaker%2C+BD&rft.au=Casey%2C+SJ&rft.au=Taupier%2C+R&rft.date=2012-04-01&rft.pub=Blackwell+Publishing+Ltd&rft.issn=0936-6768&rft.eissn=1439-0531&rft.volume=47&rft.issue=2&rft.spage=263&rft.epage=268&rft_id=info:doi/10.1111%2Fj.1439-0531.2011.01848.x&rft.externalDBID=10.1111%252Fj.1439-0531.2011.01848.x&rft.externalDocID=RDA1848 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0936-6768&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0936-6768&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0936-6768&client=summon |