Potential pitfalls of CRISPR/Cas9‐mediated genome editing

• Published in: The FEBS journal Vol. 283; no. 7; pp. 1218 - 1231
• Main Authors: Peng, Rongxue, Lin, Guigao, Li, Jinming
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.04.2016
• Subjects: Binding Sites - genetics; Biological effects; Biological evolution; Biology; Cas9; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; Design; Design engineering; Diseases; DNA; DNA cleavage; DNA damage; Editing; Efficiency; Endonuclease; Functional anatomy; Gene sequencing; gene targeting; Gene Targeting - methods; Gene therapy; Genetic Engineering - methods; Genetic Therapy - methods; genome; genome editing; Genome, Human - genetics; Genomes; Homology; Humans; Incidence; Models, Genetic; Modulation; Molecular biology; Mutation; Pathogenesis; phenotype; potential pitfalls; Proteins; Repair; Reproducibility of Results; Reviews; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - genetics; Screening; sgRNA; Strands; synthetic biology; target specificity; Technology; Transcription; transcription (genetics); target specificity; genome editing; potential pitfalls; sgRNA; gene targeting; DNA cleavage; Cas9; CRISPR/Cas systems

Recently, a novel technique named the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein (Cas)9 system has been rapidly developed. This genome editing tool has improved our ability tremendously with respect to exploring the pathogenesis of diseases and correc...