Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942

• Published in: Metabolic engineering Vol. 60; no. C; pp. 56 - 65
• Main Authors: Cheah, Yi Ern, Xu, Yao, Sacco, Sarah A., Babele, Piyoosh K., Zheng, Amy O., Johnson, Carl Hirschie, Young, Jamey D.
• Format: Journal Article
• Language: English
• Published: Belgium Elsevier Inc 01.07.2020; Elsevier
• Subjects: Acetolactate Synthase - metabolism; Aldehydes - metabolism; Amino Acids - metabolism; Cyanobacteria; Isobutyraldehyde; Metabolic Engineering; Metabolic Flux Analysis; Phosphoenolpyruvate; Phosphoenolpyruvate Carboxylase - metabolism; Photoautotrophic metabolism; Pyruvate; Pyruvate Dehydrogenase Complex - metabolism; Pyruvate Kinase - metabolism; Pyruvates - metabolism; RNA, Bacterial - biosynthesis; RNA, Bacterial - genetics; Stable isotope; Synechococcus - metabolism; Stable isotope; Isobutyraldehyde; Photoautotrophic metabolism; Metabolic flux analysis; Cyanobacteria; Pyruvate; Phosphoenolpyruvate

Isotopically nonstationary metabolic flux analysis (INST-MFA) provides a versatile platform to quantitatively assess in vivo metabolic activities of autotrophic systems. By applying INST-MFA to recombinant aldehyde-producing cyanobacteria, we identified metabolic alterations that correlated with inc...