Usefulness of the rapid determination system of viral genome sequences in human stool specimens

• Published in: Journal of virological methods Vol. 179; no. 1; pp. 256 - 260
• Main Authors: Miyoshi, Masahiro, Yoshizumi, Shima, Ishida, Setsuko, Komagome, Rika, Nagano, Hideki, Kudo, Shinichi, Okano, Motohiko
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 2012; Elsevier
• Subjects: Biological and medical sciences; Caliciviridae Infections - diagnosis; Caliciviridae Infections - virology; complementary DNA; Detection of viral genome; feces; Feces - virology; Fundamental and applied biological sciences. Psychology; gastroenteritis; Gastroenteritis - virology; genome; Genome, Viral; Humans; Microbiology; Molecular Sequence Data; Norovirus; Norovirus - genetics; Norovirus - isolation & purification; nucleotide sequences; patients; Rapid determination; reverse transcriptase polymerase chain reaction; RNA, Viral - genetics; Sequence Analysis, DNA - methods; Stool specimen; Techniques used in virology; Viral Load; Virology; Rapid determination; Norovirus; Stool specimen; Detection of viral genome; Virus; Human; Nucleotide sequence; Caliciviridae; Feces; Method; Detection; Genome

► The rapid determination system of viral genome sequences (RDV) was applied for the detection of viral genomes in human stools. ► The effect of nuclease treatment of the RDV method was examined. ► The RDV method has potential for the detection of viral genomes in stool specimens. ► The procedure wi...