Osteogenic effect of controlled released rhBMP-2 in 3D printed porous hydroxyapatite scaffold

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 141; pp. 491 - 498
• Main Authors: Wang, Hai, Wu, Gui, Zhang, Jing, Zhou, Kui, Yin, Bo, Su, Xinlin, Qiu, Guixing, Yang, Guang, Zhang, Xianglin, Zhou, Gang, Wu, Zhihong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2016
• Subjects: 3D printing; adhesion; Animals; biocompatibility; bone formation; Bone Morphogenetic Protein 2 - chemistry; Bone Morphogenetic Protein 2 - pharmacokinetics; Bone Morphogenetic Protein 2 - pharmacology; Bone Regeneration - drug effects; cell culture; Cell Survival - drug effects; Cells, Cultured; chitosan; Chitosan - chemistry; coatings; collagen; colloids; Controlled release; Delayed-Action Preparations - chemistry; Delayed-Action Preparations - pharmacokinetics; Delayed-Action Preparations - pharmacology; Drug Liberation; Durapatite - chemistry; Humans; hydroxyapatite; Hydroxyapatite (HA); in vitro studies; in vivo studies; Mesenchymal Stromal Cells - drug effects; micro-computed tomography; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Microspheres; Molecular Imprinting; Osteogenesis - drug effects; Osteogenic effect; Porosity; Rabbits; Recombinant Proteins - chemistry; Recombinant Proteins - pharmacokinetics; Recombinant Proteins - pharmacology; rhBMP-2; scanning electron microscopy; staining; Tissue Scaffolds - chemistry; Transforming Growth Factor beta - chemistry; Transforming Growth Factor beta - pharmacokinetics; Transforming Growth Factor beta - pharmacology; rhBMP-2; Hydroxyapatite (HA); Osteogenic effect; 3D printing; Controlled release

[Display omitted] •The porous HA/collagen/rhBMP-2 Microspheres delivery system was successfully fabricated.•Release kinetics of rhBMP-2 showed a biphasic profile.•Its osteoinductivity was confirmed in vitro and in vivo. Recently, 3D printing as effective technology has been highlighted in the biomed...