Overexpression of AtWRKY28 and AtWRKY75 in Arabidopsis enhances resistance to oxalic acid and Sclerotinia sclerotiorum

• Published in: Plant cell reports Vol. 32; no. 10; pp. 1589 - 1599
• Main Authors: Chen, Xiaoting, Liu, Jun, Lin, Guifang, Wang, Airong, Wang, Zonghua, Lu, Guodong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.10.2013; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - microbiology; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Ascomycota; Biomedical and Life Sciences; Biotechnology; Botrytis cinerea; Cell Biology; Cyclopentanes; Cyclopentanes - metabolism; Disease Resistance; DNA-Binding Proteins; DNA-Binding Proteins - genetics; ethylene; Ethylenes; Ethylenes - metabolism; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant; gene overexpression; genes; genetics; Host plants; jasmonic acid; Life Sciences; metabolism; microarray technology; microbiology; Original Paper; Oxalic acid; Oxalic Acid - pharmacology; Oxylipins; Oxylipins - metabolism; Pathogens; pharmacology; Plant Biochemistry; Plant Diseases; Plant Diseases - genetics; plant pathogenic fungi; Plant Sciences; Plants, Genetically Modified; Plants, Genetically Modified - genetics; Plants, Genetically Modified - microbiology; Respiratory Burst; salicylic acid; Sclerotinia sclerotiorum; transcription (genetics); transcription factors; Transcription Factors - genetics; Oxalic acid; Arabidopsis; JA; Stress responses; SA signaling; ET signaling; WRKY transcription factors
• ISSN: 0721-7714; 1432-203X; 1432-203X
• DOI: 10.1007/s00299-013-1469-3

KEY MESSAGE : Based on Arabidopsis microarray, we found 8 WRKY genes were up-regulated with Oxalic acid (OA) challenge, AtWRKY28 and AtWRKY75 overexpression lines showed enhanced resistance to OA and Sclerotinia sclerotiorum. The WRKY transcription factors are involved in various plant physiological processes and most remarkably in coping with diverse biotic and abiotic stresses. Oxalic acid (OA) is an important pathogenicity-determinant of necrotrophic phytopathogenic fungi, such as Sclerotina sclerotiorum (S. sclerotiorum) and Botrytis cinerea (B. cinerea). The identification of differentially expressed genes under OA stress should facilitate our understanding of the pathogenesis mechanism of OA-producing fungi in host plants, and the mechanism of how plants respond to OA and pathogen infection. Based on Arabidopsis oligo microarray, we found 8 WRKY genes that were up-regulated upon OA challenge. The Arabidopsis plants overexpressing AtWRKY28 and AtWRK75 showed enhanced resistance to OA and S. sclerotiorum simultaneously. Furthermore, our results showed that overexpression of AtWRKY28 and AtWRK75 induced oxidative burst in host plants, which suppressed the hyphal growth of S. sclerotiorum, and consequently inhibited fungal infection. Gene expression profiling indicates that both AtWRKY28 and AtWRKY75 are transcriptional regulators of salicylic acid (SA)- and jasmonic acid/ethylene (JA/ET)-dependent defense signaling pathways, AtWRKY28 and AtWRKY75 mainly active JA/ET pathway to defend Arabidopsis against S. sclerotiorum and oxalic acid stress.