Transcriptome-wide Identification of RNA-Binding Protein and MicroRNA Target Sites by PAR-CLIP

• Published in: Cell Vol. 141; no. 1; pp. 129 - 141
• Main Authors: Hafner, Markus, Landthaler, Markus, Burger, Lukas, Khorshid, Mohsen, Hausser, Jean, Berninger, Philipp, Rothballer, Andrea, Ascano, Manuel, Jungkamp, Anna-Carina, Munschauer, Mathias, Ulrich, Alexander, Wardle, Greg S., Dewell, Scott, Zavolan, Mihaela, Tuschl, Thomas
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.04.2010
• Subjects: Base Sequence; Cross-Linking Reagents - metabolism; Genetic Techniques; Humans; MicroRNAs - metabolism; Molecular Sequence Data; Nucleosides - metabolism; Point Mutation; PROTEINS; Regulatory Sequences, Ribonucleic Acid; RNA; RNA, Untranslated - genetics; RNA-Binding Proteins - metabolism; Sequence Alignment; SYSBIO; RNA; SYSBIO; PROTEINS

RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolutio...