Isolation and Molecular Characterization of Chitinase-Deficient Bacillus licheniformis Strains Capable of Deproteinization of Shrimp Shell Waste To Obtain Highly Viscous Chitin

• Published in: Applied and Environmental Microbiology Vol. 72; no. 12; pp. 7879 - 7885
• Main Authors: Waldeck, Jens, Daum, Gabriele, Bisping, Bernward, Meinhardt, Friedhelm
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2006
• Subjects: Acid production; Amino Acid Sequence; analysis; Animals; Bacillus; Bacillus - classification; Bacillus - enzymology; Bacillus - genetics; Bacillus - growth & development; Bacillus licheniformis; Bacteria; Bacterial Proteins; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Biological and medical sciences; Biotechnology; Biotechnology - methods; chemistry; Chitin; Chitin - metabolism; chitinase; Chitinases; Chitinases - chemistry; Chitinases - genetics; Chitinases - metabolism; classification; Decapoda (Crustacea); Decapoda - metabolism; endo-1,4-beta-glucanase; Enzymes; enzymology; Fermentation; frameshift mutation; Fundamental and applied biological sciences. Psychology; genetics; growth & development; loci; Marine; metabolism; methods; Microbiology; mixed culture; Molecular Sequence Data; Mutation; nucleotide sequences; operon; Physiology and Biotechnology; polyglutamic acid; protein content; Proteins; Proteins - analysis; Proteins - metabolism; ribosomal RNA; secretion; Sequence Analysis, DNA; Shellfish; shelling; shrimp; Viscosity; Waste Products; wastes; Chitinase; Enzyme; Bacillus licheniformis; Chitin; Bacillaceae; Deproteinization; Macrura; Crustacea; Characterization; Bacillales; Arthropoda; Glycosidases; Bacteria; Hydrolases; Isolation; Decapoda; Wastes; Invertebrata; O-Glycosidases; Shrimp

Proteolytic but chitinase-deficient microbial cultures were isolated from shrimp shell waste and characterized. The most efficient isolate was found to be a mixed culture consisting of two Bacillus licheniformis strains, which were first determined microscopically and physiologically. Molecular char...