An ELISA-based method to quantify osteocalcin carboxylation in mice

• Published in: Biochemical and biophysical research communications Vol. 397; no. 4; pp. 691 - 696
• Main Authors: Ferron, Mathieu, Wei, Jianwen, Yoshizawa, Tatsuya, Ducy, Patricia, Karsenty, Gerard
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.07.2010
• Subjects: Animals; Antibodies - immunology; Carboxylation; Cells, Cultured; Culture Media - analysis; ELISA; Enzyme-Linked Immunosorbent Assay; Mice; Mice, Mutant Strains; Osteoblasts - chemistry; Osteoblasts - drug effects; Osteocalcin; Osteocalcin - blood; Osteocalcin - immunology; Osteocalcin - metabolism; Serum; Warfarin - pharmacology; Carboxylation; Serum; Osteocalcin; ELISA

Osteocalcin was recently identified as an osteoblast-secreted hormone regulating insulin secretion and sensitivity. In mice and humans, osteocalcin can be present in the serum in carboxylated or undercarboxylated forms and it has been shown that it is the undercarboxylated form of osteocalcin which...