Der p 1 is the primary activator of Der p 3, Der p 6 and Der p 9 the proteolytic allergens produced by the house dust mite Dermatophagoides pteronyssinus

• Published in: Biochimica et biophysica acta Vol. 1840; no. 3; pp. 1117 - 1124
• Main Authors: Herman, Julie, Thelen, Nicolas, Smargiasso, Nicolas, Mailleux, Anne-Catherine, Luxen, André, Cloes, Marie, De Pauw, Edwin, Chevigné, Andy, Galleni, Moreno, Dumez, Marie-Eve
• Format: Journal Article Web Resource
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2014; Elsevier Science
• Subjects: Allergens; Allergens - metabolism; AMC; AMg; Amino Acid Sequence; Animals; Antigens, Dermatophagoides; Antigens, Dermatophagoides - analysis; Antigens, Dermatophagoides - metabolism; Arthropod Proteins; Arthropod Proteins - analysis; Arthropod Proteins - metabolism; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; cysteine; Cysteine Endopeptidases; Cysteine Endopeptidases - analysis; Cysteine Endopeptidases - metabolism; DAPI; Dermatophagoides pteronyssinus; Dermatophagoides pteronyssinus - immunology; Digestion; dust; dust mites; E-64; energy transfer; Enzyme Activation; enzyme activity; enzyme inhibition; Enzyme Precursors; Enzyme Precursors - metabolism; Forster resonance energy transfer; FRET; Förster Resonance Energy Transfer; HDM(e); hypersensitivity; Komagataella pastoris; Life sciences; midgut; Mite allergen; Molecular Sequence Data; pathogenesis; peptides; Pichia pastoris; PMg; Protease; proteinases; Proteolysis; Recombinant Proteins; Recombinant Proteins - metabolism; SBTI; Sciences du vivant; serine; Serine Endopeptidases; Serine Endopeptidases - analysis; Serine Endopeptidases - metabolism; zymogens; SBTI; Mite allergen; HDM(e); E-64; DAPI; pDp6; FP; PMg; Digestion; Dermatophagoides pteronyssinus; nDer p 6; Protease; AMC; rDer p 6; FRET; AMg; rDer p 1; Hg; Förster Resonance Energy Transfer; natural Der p 6; recombinant Der p 1; hindgut; 7-amido-4-methylcoumarin; proDer p 6; recombinant Der p 6; 4′,6-DiAmidino-2-PhenylIndole; anterior midgut; posterior midgut; soybean trypsin inhibitor; house dust mite (extracts); l-trans-epoxysuccinyl-leucylamido (4-guanidino) butane; faecal pellet

The enzymatic activity of the four proteases found in the house dust mite Dermatophagoides pteronyssinus is involved in the pathogenesis of allergy. Our aim was to elucidate the activation cascade of their corresponding precursor forms and particularly to highlight the interconnection between protea...