Subtyping of the Legionella pneumophila “Ulm” outbreak strain using the CRISPR–Cas system

• Published in: International journal of medical microbiology Vol. 305; no. 8; pp. 828 - 837
• Main Authors: Lück, Christian, Brzuszkiewicz, Elzbieta, Rydzewski, Kerstin, Koshkolda, Tetyana, Sarnow, Katharina, Essig, Andreas, Heuner, Klaus
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.12.2015
• Subjects: CRISPR-Cas Systems; CRISPR–Cas; Disease Outbreaks; Environmental Microbiology; Genetic Variation; Genomic island; Genomic Islands; Genotype; Germany - epidemiology; Humans; Infectious Disease; Legionella pneumophila - classification; Legionella pneumophila - genetics; Legionella pneumophila - isolation & purification; Legionella pneumophila Ulm; Legionnaires' Disease - epidemiology; Legionnaires' Disease - microbiology; Medical Education; Molecular Epidemiology; Outbreak; Legionella pneumophila Ulm; Outbreak; Genomic island; CRISPR–Cas

Abstract In 2009/2010 an outbreak of Legionnaires’ disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila serogroup 1, mAb type Knoxville, sequence type (ST) 62 was identified as the epidemic strain. This strain was isolated from eight patien...