Subtyping of the Legionella pneumophila “Ulm” outbreak strain using the CRISPR–Cas system
Abstract In 2009/2010 an outbreak of Legionnaires’ disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila serogroup 1, mAb type Knoxville, sequence type (ST) 62 was identified as the epidemic strain. This strain was isolated from eight patien...
Saved in:
Published in | International journal of medical microbiology Vol. 305; no. 8; pp. 828 - 837 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Elsevier GmbH
01.12.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Abstract In 2009/2010 an outbreak of Legionnaires’ disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila serogroup 1, mAb type Knoxville, sequence type (ST) 62 was identified as the epidemic strain. This strain was isolated from eight patients and from a cooling tower in the city of Ulm. Based on whole genome sequencing data from one patient strain, we identified an Lvh type IV secretion system containing a CRISPR–Cas system. The CRISPR sequence contains 38 spacer DNA sequences. We used these variable DNA spacers to further subtype the outbreak strain as well as six epidemiologically unrelated strains of CRISPR–Cas positive ST62 strains isolated at various regions in Germany. The first 12 spacer DNAs of eight patient isolates and three environmental isolates from the suspected source of infection were analyzed and found to be identical. Spacer DNAs were identified in further six epidemiologically unrelated patient isolates of L. pneumophila of ST62 in addition to the 12 “core” spacers. The presence of new spacer DNAs at the 5′ site downstream of the first repeat indicates that these CRISPR–Cas systems seem to be functional. PCR analysis revealed that not all L. pneumophila sg1 ST62 strains investigated exhibited a CRISPR–Cas system. In addition, we could demonstrate that the CRISPR–Cas system is localized on a genomic island (LpuGI-Lvh) which can be excised from the chromosome and therefore may be transferable horizontally to other L. pneumophila strains. |
---|---|
AbstractList | In 2009/2010 an outbreak of Legionnaires' disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila serogroup 1, mAb type Knoxville, sequence type (ST) 62 was identified as the epidemic strain. This strain was isolated from eight patients and from a cooling tower in the city of Ulm. Based on whole genome sequencing data from one patient strain, we identified an Lvh type IV secretion system containing a CRISPR-Cas system. The CRISPR sequence contains 38 spacer DNA sequences. We used these variable DNA spacers to further subtype the outbreak strain as well as six epidemiologically unrelated strains of CRISPR-Cas positive ST62 strains isolated at various regions in Germany. The first 12 spacer DNAs of eight patient isolates and three environmental isolates from the suspected source of infection were analyzed and found to be identical. Spacer DNAs were identified in further six epidemiologically unrelated patient isolates of L. pneumophila of ST62 in addition to the 12 "core" spacers. The presence of new spacer DNAs at the 5' site downstream of the first repeat indicates that these CRISPR-Cas systems seem to be functional. PCR analysis revealed that not all L. pneumophila sg1 ST62 strains investigated exhibited a CRISPR-Cas system. In addition, we could demonstrate that the CRISPR-Cas system is localized on a genomic island (LpuGI-Lvh) which can be excised from the chromosome and therefore may be transferable horizontally to other L. pneumophila strains. Abstract In 2009/2010 an outbreak of Legionnaires’ disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila serogroup 1, mAb type Knoxville, sequence type (ST) 62 was identified as the epidemic strain. This strain was isolated from eight patients and from a cooling tower in the city of Ulm. Based on whole genome sequencing data from one patient strain, we identified an Lvh type IV secretion system containing a CRISPR–Cas system. The CRISPR sequence contains 38 spacer DNA sequences. We used these variable DNA spacers to further subtype the outbreak strain as well as six epidemiologically unrelated strains of CRISPR–Cas positive ST62 strains isolated at various regions in Germany. The first 12 spacer DNAs of eight patient isolates and three environmental isolates from the suspected source of infection were analyzed and found to be identical. Spacer DNAs were identified in further six epidemiologically unrelated patient isolates of L. pneumophila of ST62 in addition to the 12 “core” spacers. The presence of new spacer DNAs at the 5′ site downstream of the first repeat indicates that these CRISPR–Cas systems seem to be functional. PCR analysis revealed that not all L. pneumophila sg1 ST62 strains investigated exhibited a CRISPR–Cas system. In addition, we could demonstrate that the CRISPR–Cas system is localized on a genomic island (LpuGI-Lvh) which can be excised from the chromosome and therefore may be transferable horizontally to other L. pneumophila strains. |
Author | Lück, Christian Rydzewski, Kerstin Essig, Andreas Brzuszkiewicz, Elzbieta Koshkolda, Tetyana Sarnow, Katharina Heuner, Klaus |
Author_xml | – sequence: 1 fullname: Lück, Christian – sequence: 2 fullname: Brzuszkiewicz, Elzbieta – sequence: 3 fullname: Rydzewski, Kerstin – sequence: 4 fullname: Koshkolda, Tetyana – sequence: 5 fullname: Sarnow, Katharina – sequence: 6 fullname: Essig, Andreas – sequence: 7 fullname: Heuner, Klaus |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26294350$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kctu1EAQRVsoEXnAD7BAXrKxqerX2BJCQiMIkUYKypB1q6fdTtrxi2470uzmH7JNfm6-hLYmsGDBqmpx7y3VuWfkqOs7S8g7hAwB5cc6c3XbZhRQZJBnAPiKnKLEPAUJi6O4c5annFI8IWch1ABACyZfkxMqacGZgFOi1tNm3A6uu036KhnvbLKyty7eaRqdDJ2d2n64c3Hf755umna_e076adx4q--TMHrtumQKs3u2Lq8v1z-u97vHpQ5J2IbRtm_IcaWbYN--zHNy8-3rz-X3dHV1cbn8skqNEDim5cYwjmWpF5IXBi0tZFVVOZciZxSMFJQxjbqIasGw0MA0BckNE1IiwoKdkw-H3MH3vyYbRtW6YOYvOttPQeFCAKeCo4hSepAa34fgbaUG71rttwpBzWBVrWawagarIFcRbDS9f8mfNq0t_1r-kIyCTweBjV8-OOtVMM52xpbOWzOqsnf_z__8j900rnNGN_d2a0PdT76L_BSqQBWo9Vzt3CyK2KqknP0G0KahuA |
CitedBy_id | crossref_primary_10_1016_j_idc_2016_10_012 crossref_primary_10_1016_j_ijmm_2016_01_001 crossref_primary_10_1099_jmm_0_000257 crossref_primary_10_1128_iai_00369_22 crossref_primary_10_1111_cmi_12586 crossref_primary_10_1016_j_meegid_2016_04_033 crossref_primary_10_3389_fmicb_2017_02036 crossref_primary_10_3390_microorganisms11030738 crossref_primary_10_1051_medsci_2018215 crossref_primary_10_1371_journal_pone_0164074 crossref_primary_10_1128_iai_00229_23 crossref_primary_10_1002_1873_3468_12326 |
Cites_doi | 10.1099/00222615-48-4-327 10.1128/AEM.41.1.9-16.1981 10.1126/science.1099776 10.1136/jcp.33.12.1179 10.1186/1745-6150-6-38 10.3389/fmicb.2011.00208 10.1128/CMR.15.3.506-526.2002 10.1016/j.ijmm.2013.07.003 10.1111/j.1462-5822.2006.00703.x 10.1038/nrmicro2577 10.1128/JB.00778-10 10.1128/JCM.00898-07 10.1093/nar/gks216 10.1128/JCM.00219-14 10.1186/1471-2164-12-536 10.1371/journal.pgen.1000851 10.1016/j.ijmm.2007.07.012 10.1128/mBio.01091-14 10.1099/ijs.0.068940-0 10.1126/science.1138140 10.1128/AEM.71.1.282-289.2005 10.1371/journal.pone.0103852 10.1017/S0022172400063877 10.1128/JB.01739-12 10.1086/341087 10.1056/NEJM197712012972201 10.1186/1471-2164-11-181 10.1099/mic.0.29227-0 10.1128/IAI.03102-14 10.2807/ese.15.04.19472-en 10.1128/mBio.00074-13 10.1038/nrmicro1793 10.1038/ng1447 10.1128/JCM.14.3.298-303.1981 10.7326/0003-4819-90-4-656 10.1007/s10096-002-0820-3 10.1186/1471-2180-13-198 10.1017/S0022172400062252 10.1128/JCM.35.4.907-914.1997 10.1128/JCM.37.8.2652-2655.1999 10.1371/journal.pone.0082221 10.1016/0966-842X(96)10041-X 10.1128/JCM.06180-11 10.1017/S0022172400029090 10.3201/eid1404.070961 10.1038/nature10886 10.1093/bioinformatics/btu153 10.1101/gr.7229808 10.1111/j.1365-2672.1988.tb01501.x 10.1093/bioinformatics/btp393 10.1128/CMR.00029-14 10.1016/S0011-5029(80)80010-1 |
ContentType | Journal Article |
Copyright | Elsevier GmbH 2015 Elsevier GmbH Copyright © 2015 Elsevier GmbH. All rights reserved. |
Copyright_xml | – notice: Elsevier GmbH – notice: 2015 Elsevier GmbH – notice: Copyright © 2015 Elsevier GmbH. All rights reserved. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1016/j.ijmm.2015.08.001 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1618-0607 |
EndPage | 837 |
ExternalDocumentID | 10_1016_j_ijmm_2015_08_001 26294350 S1438422115000624 1_s2_0_S1438422115000624 |
Genre | Journal Article |
GroupedDBID | --- --K --M .1- .FO .~1 0R~ 0SF 1B1 1P~ 1~. 1~5 29J 3O- 3V. 4.4 457 4G. 53G 5GY 5VS 7-5 71M 7X7 88A 88E 88I 8AF 8C1 8CJ 8FE 8FH 8FI 8FJ 8P~ 8R4 8R5 AAAJQ AABNK AACTN AAEDT AAEDW AAFWJ AAIKJ AAKOC AAKPP AALRI AAOAW AAQFI AAQXK AARKO AAXKI AAXUO ABBQC ABFNM ABFRF ABGSF ABJNI ABKYH ABMAC ABMZM ABOHT ABRWV ABUDA ABUWG ABXDB ABZDS ACDAQ ACGFO ACGFS ACGOD ACPRK ACRLP ADBBV ADEZE ADKUU ADMUD ADUVX ADVLN AEBSH AEFWE AEHWI AEKER AENEX AEVXI AEXOQ AFCTW AFJKZ AFKRA AFKWA AFPKN AFRHN AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHMBA AI. AIEXJ AIKHN AITUG AJOXV AJRQY AJUYK AKRWK ALIPV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN AZQEC BBNVY BENPR BES BHPHI BKOJK BLXMC BNPGV BPHCQ BVXVI CAG CCPQU CJTIS COF CS3 D1J DU5 DWQXO EBS EFJIC EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN FYUFA G-Q GBLVA GNUQQ GROUPED_DOAJ HCIFZ HVGLF HZ~ IHE J1W KOM LK8 LUGTX M0L M1P M2P M2Q M41 M7P MO0 N9A NCXOZ O-L O9- OAUVE OD- OGGZJ OK1 OO. OZT P-8 P-9 P2P PC. PQQKQ PROAC PSQYO Q2X Q38 R2- RIG ROL RPZ S0X SDF SDG SES SEW SNL SPCBC SSH SSI SSU SSZ T5F T5I T5J T5K UNMZH VH1 Z5R ~G- AAIAV ABLVK ABYKQ AESVU AJBFU DOVZS EFLBG HMCUK LCYCR QYZTP UKHRP CGR CUY CVF ECM EIF NPM AAYXX ACRPL ADNMO CITATION 7X8 |
ID | FETCH-LOGICAL-c551t-dbc341dda7649c1e296fff84658320c65233a1a9c555319a03a2064c356611073 |
IEDL.DBID | AIKHN |
ISSN | 1438-4221 |
IngestDate | Sat Oct 05 04:54:11 EDT 2024 Fri Dec 06 05:48:26 EST 2024 Sat Sep 28 08:04:55 EDT 2024 Fri Feb 23 02:27:46 EST 2024 Tue Oct 15 22:58:02 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 8 |
Keywords | Legionella pneumophila Ulm Outbreak Genomic island CRISPR–Cas |
Language | English |
License | Copyright © 2015 Elsevier GmbH. All rights reserved. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c551t-dbc341dda7649c1e296fff84658320c65233a1a9c555319a03a2064c356611073 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 26294350 |
PQID | 1750425415 |
PQPubID | 23479 |
PageCount | 10 |
ParticipantIDs | proquest_miscellaneous_1750425415 crossref_primary_10_1016_j_ijmm_2015_08_001 pubmed_primary_26294350 elsevier_sciencedirect_doi_10_1016_j_ijmm_2015_08_001 elsevier_clinicalkeyesjournals_1_s2_0_S1438422115000624 |
PublicationCentury | 2000 |
PublicationDate | 2015-12-01 |
PublicationDateYYYYMMDD | 2015-12-01 |
PublicationDate_xml | – month: 12 year: 2015 text: 2015-12-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Germany |
PublicationPlace_xml | – name: Germany |
PublicationTitle | International journal of medical microbiology |
PublicationTitleAlternate | Int J Med Microbiol |
PublicationYear | 2015 |
Publisher | Elsevier GmbH |
Publisher_xml | – name: Elsevier GmbH |
References | Fields, Benson, Besser (bib0080) 2002; 15 Seemann (bib0250) 2014; 30 Fraser, Tsai, Orenstein, Parkin, Beecham, Sharrar, Harris, Mallison, Martin, McDade, Shepard, Brachman (bib0100) 1977; 297 Gunderson, Mallama, Fairbairn, Cianciotto (bib0140) 2015; 83 Chien, Morozova, Shi, Sheng, Chen, Gomez, Asamani, Hill, Nuara, Feder, Rineer, Greenberg, Steshenko, Park, Zhao, Teplitskaya, Edwards, Pampou, Georghiou, Chou, Iannuccilli, Ulz, Kim, Geringer-Sameth, Goldsberry, Morozov, Fischer, Segal, Qu, Rzhetsky, Zhang, Cayanis, De Jong, Ju, Kalachikov, Shuman, Russo (bib0050) 2004; 305 Flynn, Swanson (bib0095) 2014; 5 Watanabe, Suzuki, Nakao, Shimizu, Watarai (bib0270) 2015 von Baum, Harter, Essig, Luck, Gonser, Embacher, Brockmann (bib0265) 2010; 15 McAdam, Vander Broek, Lindsay, Ward, Hanson, Gillies, Watson, Stevens, Edwards, Fitzgerald (bib0205) 2014; 15 Glöckner, Albert-Weissenberger, Weinmann, Jacobi, Schunder, Steinert, Hacker, Heuner (bib0115) 2008; 298 Mercante, Winchell (bib0210) 2015; 28 Dennis, Lee (bib0060) 1988; 65 Brenner, Steigerwalt, McDade (bib0020) 1979; 90 Fields (bib0075) 1996; 4 Fitzgeorge, Baskerville, Broster, Hambleton, Dennis (bib0085) 1983; 90 Wee, Woolfit, Beatson, Petty (bib0275) 2013; 8 Aurell, Farge, Meugnier, Gouy, Forey, Lina, Vandenesch, Etienne, Jarraud (bib0010) 2005; 71 Levesque, Plante, Mendis, Cantin, Marchand, Charest, Raymond, Huot, Goupil-Sormany, Desbiens, Faucher, Corbeil, Tremblay (bib0185) 2014; 9 Schroeder, Petty, Mousnier, Harding, Vogrin, Wee, Fry, Harrison, Newton, Thomson, Beatson, Dougan, Hartland, Frankel (bib0245) 2010; 192 D’Auria, Jimenez-Hernandez, Peris-Bondia, Moya, Latorre (bib0055) 2010; 11 Doleans-Jordheim, Akermi, Ginevra, Cazalet, Kay, Schneider, Buchrieser, Atlan, Vandenesch, Etienne, Jarraud (bib0065) 2006; 152 Jepras, Fitzgeorge, Baskerville (bib0155) 1985; 95 Cazalet, Jarraud, Ghavi-Helm, Kunst, Glaser, Etienne, Buchrieser (bib0040) 2008; 18 Petzold, Thurmer, Menzel, Mouton, Heuner, Luck (bib0225) 2013; 13 Sorek, Kunin, Hugenholtz (bib0260) 2008; 6 Markowitz, Mavromatis, Ivanova, Chen, Chu, Kyrpides (bib0200) 2009; 25 Gomez-Valero, Rusniok, Cazalet, Buchrieser (bib0120) 2011; 2 Shands, Fraser (bib0255) 1980; 27 Ginevra, Forey, Campese, Reyrolle, Che, Etienne, Jarraud (bib0105) 2008; 14 Yu, Plouffe, Pastoris, Stout, Schousboe, Widmer, Summersgill, File, Heath, Paterson, Chereshsky (bib0290) 2002; 186 Lee, West (bib0180) 1991; 20 O'Shaughnessy, Chan, Clark, Ivanetich (bib0220) 2003; 35 Brzuszkiewicz, Schulz, Rydzewski, Daniel, Gillmaier, Dittmann, Holland, Schunder, Lautner, Eisenreich, Luck, Heuner (bib0030) 2013; 303 Kamerbeek, Schouls, Kolk, van Agterveld, van Soolingen, Kuijper, Bunschoten, Molhuizen, Shaw, Goyal, van Embden (bib0160) 1997; 35 Gomgnimbou, Ginevra, Peron-Cane, Versapuech, Refregier, Jacotin, Sola, Jarraud (bib0130) 2014; 52 Barrangou, Fremaux, Deveau, Richards, Boyaval, Moineau, Romero, Horvath (bib0015) 2007; 315 Fliermans, Cherry, Orrison, Smith, Tison, Pope (bib0090) 1981; 41 Lawrence, Reyrolle, Dubrou, Forey, Decludt, Goulvestre, Matsiota-Bernard, Etienne, Nauciel (bib0170) 1999; 37 Cazalet, Gomez-Valero, Rusniok, Lomma, Dervins-Ravault, Newton, Sansom, Jarraud, Zidane, Ma, Bouchier, Etienne, Hartland, Buchrieser (bib0035) 2010; 6 Gomez-Valero, Rusniok, Jarraud, Vacherie, Rouy, Barbe, Medigue, Etienne, Buchrieser (bib0125) 2011; 12 Ginevra, Jacotin, Diancourt, Guigon, Arquilliere, Meugnier, Descours, Vandenesch, Etienne, Lina, Caro, Jarraud (bib0110) 2012; 50 Brüggemann, Hagman, Jules, Sismeiro, Dillies, Gouyette, Kunst, Steinert, Heuner, Coppee, Buchrieser (bib0025) 2006; 8 Helbig, Bernander, Castellani Pastoris, Etienne, Gaia, Lauwers, Lindsay, Luck, Marques, Mentula, Peeters, Pelaz, Struelens, Uldum, Wewalka, Harrison (bib0150) 2002; 21 Afshar, Fry, Bellamy, Underwood, Harrison, Members of the European Working Group for Legionella I (bib0005) 2007; 45 Rowbotham (bib0235) 1980; 33 Morrison, Kozak-Muiznieks, Sammons, Rowe, Frace, Winchell (bib0215) 2015 Wiedenheft, Sternberg, Doudna (bib0280) 2012; 482 Edelstein (bib0070) 1981; 14 Rowbotham (bib0240) 1986; 22 Gunderson, Cianciotto (bib0135) 2013; 4 Makarova, Haft, Barrangou, Brouns, Charpentier, Horvath, Moineau, Mojica, Wolf, Yakunin, van der Oost, Koonin (bib0195) 2011; 9 Cazalet, Rusniok, Brüggemann, Zidane, Magnier, Ma, Tichit, Jarraud, Bouchier, Vandenesch, Kunst, Etienne, Glaser, Buchrieser (bib0045) 2004; 36 Makarova, Aravind, Wolf, Koonin (bib0190) 2011; 6 Yosef, Goren, Qimron (bib0285) 2012; 40 Rizzardi, Winiecka-Krusnell, Ramliden, Alm, Andersson, Byfors (bib0230) 2015; 65 Lawrence, Ronco, Dubrou, Leclercq, Nauciel, Matsiota-Bernard (bib0175) 1999; 48 Hambleton, Broster, Dennis, Henstridge, Fitzgeorge, Conlan (bib0145) 1983; 90 Lautner, Schunder, Herrmann, Heuner (bib0165) 2013; 195 Chien (10.1016/j.ijmm.2015.08.001_bib0050) 2004; 305 Gunderson (10.1016/j.ijmm.2015.08.001_bib0140) 2015; 83 Jepras (10.1016/j.ijmm.2015.08.001_bib0155) 1985; 95 Edelstein (10.1016/j.ijmm.2015.08.001_bib0070) 1981; 14 McAdam (10.1016/j.ijmm.2015.08.001_bib0205) 2014; 15 Fields (10.1016/j.ijmm.2015.08.001_bib0080) 2002; 15 Gomgnimbou (10.1016/j.ijmm.2015.08.001_bib0130) 2014; 52 Wee (10.1016/j.ijmm.2015.08.001_bib0275) 2013; 8 Markowitz (10.1016/j.ijmm.2015.08.001_bib0200) 2009; 25 Dennis (10.1016/j.ijmm.2015.08.001_bib0060) 1988; 65 Brenner (10.1016/j.ijmm.2015.08.001_bib0020) 1979; 90 Cazalet (10.1016/j.ijmm.2015.08.001_bib0045) 2004; 36 Morrison (10.1016/j.ijmm.2015.08.001_bib0215) 2015 Schroeder (10.1016/j.ijmm.2015.08.001_bib0245) 2010; 192 Kamerbeek (10.1016/j.ijmm.2015.08.001_bib0160) 1997; 35 Rizzardi (10.1016/j.ijmm.2015.08.001_bib0230) 2015; 65 Lawrence (10.1016/j.ijmm.2015.08.001_bib0170) 1999; 37 Fraser (10.1016/j.ijmm.2015.08.001_bib0100) 1977; 297 Yu (10.1016/j.ijmm.2015.08.001_bib0290) 2002; 186 Aurell (10.1016/j.ijmm.2015.08.001_bib0010) 2005; 71 Wiedenheft (10.1016/j.ijmm.2015.08.001_bib0280) 2012; 482 Glöckner (10.1016/j.ijmm.2015.08.001_bib0115) 2008; 298 Afshar (10.1016/j.ijmm.2015.08.001_bib0005) 2007; 45 Lautner (10.1016/j.ijmm.2015.08.001_bib0165) 2013; 195 Ginevra (10.1016/j.ijmm.2015.08.001_bib0110) 2012; 50 Lawrence (10.1016/j.ijmm.2015.08.001_bib0175) 1999; 48 Flynn (10.1016/j.ijmm.2015.08.001_bib0095) 2014; 5 Lee (10.1016/j.ijmm.2015.08.001_bib0180) 1991; 20 von Baum (10.1016/j.ijmm.2015.08.001_bib0265) 2010; 15 Fitzgeorge (10.1016/j.ijmm.2015.08.001_bib0085) 1983; 90 Gomez-Valero (10.1016/j.ijmm.2015.08.001_bib0125) 2011; 12 Mercante (10.1016/j.ijmm.2015.08.001_bib0210) 2015; 28 Rowbotham (10.1016/j.ijmm.2015.08.001_bib0240) 1986; 22 Fliermans (10.1016/j.ijmm.2015.08.001_bib0090) 1981; 41 Cazalet (10.1016/j.ijmm.2015.08.001_bib0040) 2008; 18 Fields (10.1016/j.ijmm.2015.08.001_bib0075) 1996; 4 Seemann (10.1016/j.ijmm.2015.08.001_bib0250) 2014; 30 D’Auria (10.1016/j.ijmm.2015.08.001_bib0055) 2010; 11 Sorek (10.1016/j.ijmm.2015.08.001_bib0260) 2008; 6 Brüggemann (10.1016/j.ijmm.2015.08.001_bib0025) 2006; 8 Cazalet (10.1016/j.ijmm.2015.08.001_bib0035) 2010; 6 Barrangou (10.1016/j.ijmm.2015.08.001_bib0015) 2007; 315 Doleans-Jordheim (10.1016/j.ijmm.2015.08.001_bib0065) 2006; 152 Gomez-Valero (10.1016/j.ijmm.2015.08.001_bib0120) 2011; 2 Rowbotham (10.1016/j.ijmm.2015.08.001_bib0235) 1980; 33 O'Shaughnessy (10.1016/j.ijmm.2015.08.001_bib0220) 2003; 35 Ginevra (10.1016/j.ijmm.2015.08.001_bib0105) 2008; 14 Gunderson (10.1016/j.ijmm.2015.08.001_bib0135) 2013; 4 Makarova (10.1016/j.ijmm.2015.08.001_bib0190) 2011; 6 Watanabe (10.1016/j.ijmm.2015.08.001_bib0270) 2015 Helbig (10.1016/j.ijmm.2015.08.001_bib0150) 2002; 21 Yosef (10.1016/j.ijmm.2015.08.001_bib0285) 2012; 40 Brzuszkiewicz (10.1016/j.ijmm.2015.08.001_bib0030) 2013; 303 Hambleton (10.1016/j.ijmm.2015.08.001_bib0145) 1983; 90 Petzold (10.1016/j.ijmm.2015.08.001_bib0225) 2013; 13 Levesque (10.1016/j.ijmm.2015.08.001_bib0185) 2014; 9 Shands (10.1016/j.ijmm.2015.08.001_bib0255) 1980; 27 Makarova (10.1016/j.ijmm.2015.08.001_bib0195) 2011; 9 |
References_xml | – volume: 6 start-page: 38 year: 2011 ident: bib0190 article-title: Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR–Cas systems publication-title: Biol. Direct. contributor: fullname: Koonin – volume: 4 start-page: 286 year: 1996 end-page: 290 ident: bib0075 article-title: The molecular ecology of legionellae publication-title: Trends Microbiol. contributor: fullname: Fields – volume: 186 start-page: 127 year: 2002 end-page: 128 ident: bib0290 article-title: Distribution of publication-title: J. Infect. Dis. contributor: fullname: Chereshsky – volume: 21 start-page: 710 year: 2002 end-page: 716 ident: bib0150 article-title: Pan-European study on culture-proven Legionnaires’ disease: distribution of publication-title: Eur. J. Clin. Microbiol. Infect. Dis. contributor: fullname: Harrison – volume: 35 start-page: 118 year: 2003 end-page: 121 ident: bib0220 article-title: Primer design for automated DNA sequencing in a core facility publication-title: Biotechniques contributor: fullname: Ivanetich – volume: 6 start-page: e1000851 year: 2010 ident: bib0035 article-title: Analysis of the publication-title: PLoS Genet contributor: fullname: Buchrieser – volume: 14 start-page: 298 year: 1981 end-page: 303 ident: bib0070 article-title: Improved semiselective medium for isolation of publication-title: J. Clin. Microbiol. contributor: fullname: Edelstein – volume: 303 start-page: 514 year: 2013 end-page: 528 ident: bib0030 article-title: ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae publication-title: Int. J. Med. Microbiol. contributor: fullname: Heuner – volume: 192 start-page: 6001 year: 2010 end-page: 6016 ident: bib0245 article-title: strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins publication-title: J. Bacteriol. contributor: fullname: Frankel – volume: 83 start-page: 1008 year: 2015 end-page: 1018 ident: bib0140 article-title: Nuclease activity of publication-title: Infect. Immun. contributor: fullname: Cianciotto – volume: 13 start-page: 198 year: 2013 ident: bib0225 article-title: A structural comparison of lipopolysaccharide biosynthesis loci of publication-title: BMC Microbiol. contributor: fullname: Luck – volume: 298 start-page: 411 year: 2008 end-page: 428 ident: bib0115 article-title: Identification and characterization of a new conjugation/type IVA secretion system (trb/tra) of publication-title: Int. J. Med. Microbiol. contributor: fullname: Heuner – volume: 20 start-page: 121S year: 1991 end-page: 129S ident: bib0180 article-title: Survival and growth of publication-title: Soc. Appl. Bacteriol. Symp. Ser. contributor: fullname: West – volume: 15 start-page: 19472 year: 2010 ident: bib0265 article-title: Preliminary report: outbreak of Legionnaires disease in the cities of Ulm and Neu-Ulm in Germany December 2009–January 2010 publication-title: Eur. Surveill. contributor: fullname: Brockmann – volume: 9 start-page: e103852 year: 2014 ident: bib0185 article-title: Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012 publication-title: PLOS ONE contributor: fullname: Tremblay – volume: 41 start-page: 9 year: 1981 end-page: 16 ident: bib0090 article-title: Ecological distribution of publication-title: Appl. Environ. Microbiol. contributor: fullname: Pope – volume: 36 start-page: 1165 year: 2004 end-page: 1173 ident: bib0045 article-title: Evidence in the publication-title: Nat. Genet. contributor: fullname: Buchrieser – volume: 195 start-page: 1583 year: 2013 end-page: 1597 ident: bib0165 article-title: Regulation, integrase-dependent excision, and horizontal transfer of genomic islands in publication-title: J. Bacteriol. contributor: fullname: Heuner – volume: 2 start-page: 208 year: 2011 ident: bib0120 article-title: Comparative and functional genomics of publication-title: Front. Microbiol. contributor: fullname: Buchrieser – volume: 65 start-page: 598 year: 2015 end-page: 603 ident: bib0230 article-title: sp. nov., isolated from the biopurification systems of wood processing plants publication-title: Int. J. Syst. Evol. Microbiol. contributor: fullname: Byfors – volume: 30 start-page: 2068 year: 2014 end-page: 2069 ident: bib0250 article-title: Prokka: rapid prokaryotic genome annotation publication-title: Bioinformatics contributor: fullname: Seemann – volume: 6 start-page: 181 year: 2008 end-page: 186 ident: bib0260 article-title: CRISPR – a widespread system that provides acquired resistance against phages in bacteria and archaea publication-title: Nat. Rev. Microbiol. contributor: fullname: Hugenholtz – volume: 482 start-page: 331 year: 2012 end-page: 338 ident: bib0280 article-title: RNA-guided genetic silencing systems in bacteria and archaea publication-title: Nature contributor: fullname: Doudna – volume: 45 start-page: 3251 year: 2007 end-page: 3256 ident: bib0005 article-title: External quality assessment of a DNA sequence-based scheme for epidemiological typing of publication-title: J. Clin. Microbiol. contributor: fullname: Members of the European Working Group for Legionella I – volume: 305 start-page: 1966 year: 2004 end-page: 1968 ident: bib0050 article-title: The genomic sequence of the accidental pathogen publication-title: Science contributor: fullname: Russo – volume: 90 start-page: 81 year: 1983 end-page: 89 ident: bib0085 article-title: Aerosol infection of animals with strains publication-title: J. Hyg. (Lond.) contributor: fullname: Dennis – volume: 4 year: 2013 ident: bib0135 article-title: The CRISPR-associated gene cas2 of publication-title: MBio contributor: fullname: Cianciotto – volume: 11 start-page: 181 year: 2010 ident: bib0055 article-title: pangenome reveals strain-specific virulence factors publication-title: BMC Genomics contributor: fullname: Latorre – volume: 90 start-page: 451 year: 1983 end-page: 460 ident: bib0145 article-title: Survival of virulent publication-title: J. Hyg. (Lond.) contributor: fullname: Conlan – volume: 71 start-page: 282 year: 2005 end-page: 289 ident: bib0010 article-title: Clinical and environmental isolates of publication-title: Appl. Environ. Microbiol. contributor: fullname: Jarraud – volume: 15 start-page: 506 year: 2002 end-page: 526 ident: bib0080 article-title: and Legionnaires’ disease: 25 years of investigation publication-title: Clin. Microbiol. Rev. contributor: fullname: Besser – volume: 65 start-page: 135 year: 1988 end-page: 141 ident: bib0060 article-title: Differences in aerosol survival between pathogenic and non-pathogenic strains of publication-title: J. Appl. Bacteriol. contributor: fullname: Lee – volume: 48 start-page: 327 year: 1999 end-page: 333 ident: bib0175 article-title: Molecular typing of publication-title: J. Med. Microbiol. contributor: fullname: Matsiota-Bernard – start-page: 3 year: 2015 ident: bib0270 article-title: Draft genome sequences of five publication-title: Genome Announc. contributor: fullname: Watarai – volume: 33 start-page: 1179 year: 1980 end-page: 1183 ident: bib0235 article-title: Preliminary report on the pathogenicity of publication-title: J. Clin. Pathol. contributor: fullname: Rowbotham – volume: 22 start-page: 678 year: 1986 end-page: 689 ident: bib0240 article-title: Current views on the relationships between amoebae, legionellae and man publication-title: Isr. J. Med. Sci. contributor: fullname: Rowbotham – volume: 8 start-page: e82221 year: 2013 ident: bib0275 article-title: A distinct and divergent lineage of genomic island-associated type IV secretion systems in publication-title: PLOS ONE contributor: fullname: Petty – volume: 15 start-page: 504 year: 2014 ident: bib0205 article-title: Gene flow in environmental publication-title: Genome Biol. contributor: fullname: Fitzgerald – start-page: 3 year: 2015 ident: bib0215 article-title: Draft genome sequence of publication-title: Genome Announc. contributor: fullname: Winchell – volume: 90 start-page: 656 year: 1979 end-page: 658 ident: bib0020 article-title: Classification of the Legionnaires’ disease bacterium: publication-title: Ann. Intern. Med. contributor: fullname: McDade – volume: 14 start-page: 673 year: 2008 end-page: 675 ident: bib0105 article-title: Lorraine strain of publication-title: France. Emerg. Infect. Dis. contributor: fullname: Jarraud – volume: 5 year: 2014 ident: bib0095 article-title: Integrative conjugative element ICE-betaox confers oxidative stress resistance to publication-title: MBio contributor: fullname: Swanson – volume: 18 start-page: 431 year: 2008 end-page: 441 ident: bib0040 article-title: Multigenome analysis identifies a worldwide distributed epidemic publication-title: Genome Res. contributor: fullname: Buchrieser – volume: 40 start-page: 5569 year: 2012 end-page: 5576 ident: bib0285 article-title: Proteins and DNA elements essential for the CRISPR adaptation process in publication-title: Nucleic Acids Res. contributor: fullname: Qimron – volume: 27 start-page: 1 year: 1980 end-page: 59 ident: bib0255 article-title: Legionnaires’ disease publication-title: Dis. Mon. contributor: fullname: Fraser – volume: 315 start-page: 1709 year: 2007 end-page: 1712 ident: bib0015 article-title: CRISPR provides acquired resistance against viruses in prokaryotes publication-title: Science contributor: fullname: Horvath – volume: 8 start-page: 1228 year: 2006 end-page: 1240 ident: bib0025 article-title: Virulence strategies for infecting phagocytes deduced from the in vivo transcriptional program of publication-title: Cell. Microbiol. contributor: fullname: Buchrieser – volume: 297 start-page: 1189 year: 1977 end-page: 1197 ident: bib0100 article-title: Legionnaires’ disease: description of an epidemic of pneumonia publication-title: N. Engl. J. Med. contributor: fullname: Brachman – volume: 35 start-page: 907 year: 1997 end-page: 914 ident: bib0160 article-title: Simultaneous detection and strain differentiation of publication-title: J. Clin. Microbiol. contributor: fullname: van Embden – volume: 152 start-page: 3561 year: 2006 end-page: 3568 ident: bib0065 article-title: Growth-phase-dependent mobility of the publication-title: Microbiology contributor: fullname: Jarraud – volume: 50 start-page: 696 year: 2012 end-page: 701 ident: bib0110 article-title: sequence type 1/Paris pulsotype subtyping by spoligotyping publication-title: J. Clin. Microbiol. contributor: fullname: Jarraud – volume: 25 start-page: 2271 year: 2009 end-page: 2278 ident: bib0200 article-title: IMG ER: a system for microbial genome annotation expert review and curation publication-title: Bioinformatics contributor: fullname: Kyrpides – volume: 37 start-page: 2652 year: 1999 end-page: 2655 ident: bib0170 article-title: Single clonal origin of a high proportion of publication-title: J. Clin. Microbiol. contributor: fullname: Nauciel – volume: 95 start-page: 29 year: 1985 end-page: 38 ident: bib0155 article-title: A comparison of virulence of two strains of publication-title: J. Hyg. (Lond.) contributor: fullname: Baskerville – volume: 9 start-page: 467 year: 2011 end-page: 477 ident: bib0195 article-title: Evolution and classification of the CRISPR–Cas systems publication-title: Nat. Rev. Microbiol. contributor: fullname: Koonin – volume: 12 start-page: 536 year: 2011 ident: bib0125 article-title: Extensive recombination events and horizontal gene transfer shaped the publication-title: BMC Genomics contributor: fullname: Buchrieser – volume: 52 start-page: 2410 year: 2014 end-page: 2415 ident: bib0130 article-title: Validation of a microbead-based format for spoligotyping of publication-title: J. Clin. Microbiol. contributor: fullname: Jarraud – volume: 28 start-page: 95 year: 2015 end-page: 133 ident: bib0210 article-title: Current and emerging publication-title: Clin. Microbiol. Rev. contributor: fullname: Winchell – volume: 48 start-page: 327 year: 1999 ident: 10.1016/j.ijmm.2015.08.001_bib0175 article-title: Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis publication-title: J. Med. Microbiol. doi: 10.1099/00222615-48-4-327 contributor: fullname: Lawrence – volume: 41 start-page: 9 year: 1981 ident: 10.1016/j.ijmm.2015.08.001_bib0090 article-title: Ecological distribution of Legionella pneumophila publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.41.1.9-16.1981 contributor: fullname: Fliermans – volume: 305 start-page: 1966 year: 2004 ident: 10.1016/j.ijmm.2015.08.001_bib0050 article-title: The genomic sequence of the accidental pathogen Legionella pneumophila publication-title: Science doi: 10.1126/science.1099776 contributor: fullname: Chien – volume: 33 start-page: 1179 year: 1980 ident: 10.1016/j.ijmm.2015.08.001_bib0235 article-title: Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae publication-title: J. Clin. Pathol. doi: 10.1136/jcp.33.12.1179 contributor: fullname: Rowbotham – volume: 6 start-page: 38 year: 2011 ident: 10.1016/j.ijmm.2015.08.001_bib0190 article-title: Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR–Cas systems publication-title: Biol. Direct. doi: 10.1186/1745-6150-6-38 contributor: fullname: Makarova – start-page: 3 year: 2015 ident: 10.1016/j.ijmm.2015.08.001_bib0215 article-title: Draft genome sequence of Legionella pneumophila D-5864, a serogroup 6 strain publication-title: Genome Announc. contributor: fullname: Morrison – volume: 2 start-page: 208 year: 2011 ident: 10.1016/j.ijmm.2015.08.001_bib0120 article-title: Comparative and functional genomics of Legionella identified eukaryotic like proteins as key players in host–pathogen interactions publication-title: Front. Microbiol. doi: 10.3389/fmicb.2011.00208 contributor: fullname: Gomez-Valero – volume: 15 start-page: 506 year: 2002 ident: 10.1016/j.ijmm.2015.08.001_bib0080 article-title: Legionella and Legionnaires’ disease: 25 years of investigation publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.15.3.506-526.2002 contributor: fullname: Fields – volume: 303 start-page: 514 year: 2013 ident: 10.1016/j.ijmm.2015.08.001_bib0030 article-title: Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae publication-title: Int. J. Med. Microbiol. doi: 10.1016/j.ijmm.2013.07.003 contributor: fullname: Brzuszkiewicz – volume: 8 start-page: 1228 year: 2006 ident: 10.1016/j.ijmm.2015.08.001_bib0025 article-title: Virulence strategies for infecting phagocytes deduced from the in vivo transcriptional program of Legionella pneumophila publication-title: Cell. Microbiol. doi: 10.1111/j.1462-5822.2006.00703.x contributor: fullname: Brüggemann – volume: 9 start-page: 467 year: 2011 ident: 10.1016/j.ijmm.2015.08.001_bib0195 article-title: Evolution and classification of the CRISPR–Cas systems publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2577 contributor: fullname: Makarova – volume: 192 start-page: 6001 year: 2010 ident: 10.1016/j.ijmm.2015.08.001_bib0245 article-title: Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins publication-title: J. Bacteriol. doi: 10.1128/JB.00778-10 contributor: fullname: Schroeder – volume: 45 start-page: 3251 year: 2007 ident: 10.1016/j.ijmm.2015.08.001_bib0005 article-title: External quality assessment of a DNA sequence-based scheme for epidemiological typing of Legionella pneumophila by an international network of laboratories publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.00898-07 contributor: fullname: Afshar – volume: 40 start-page: 5569 year: 2012 ident: 10.1016/j.ijmm.2015.08.001_bib0285 article-title: Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli publication-title: Nucleic Acids Res. doi: 10.1093/nar/gks216 contributor: fullname: Yosef – volume: 52 start-page: 2410 year: 2014 ident: 10.1016/j.ijmm.2015.08.001_bib0130 article-title: Validation of a microbead-based format for spoligotyping of Legionella pneumophila publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.00219-14 contributor: fullname: Gomgnimbou – volume: 12 start-page: 536 year: 2011 ident: 10.1016/j.ijmm.2015.08.001_bib0125 article-title: Extensive recombination events and horizontal gene transfer shaped the Legionella pneumophila genomes publication-title: BMC Genomics doi: 10.1186/1471-2164-12-536 contributor: fullname: Gomez-Valero – volume: 6 start-page: e1000851 year: 2010 ident: 10.1016/j.ijmm.2015.08.001_bib0035 article-title: Analysis of the Legionella longbeachae genome and transcriptome uncovers unique strategies to cause Legionnaires’ disease publication-title: PLoS Genet doi: 10.1371/journal.pgen.1000851 contributor: fullname: Cazalet – volume: 298 start-page: 411 year: 2008 ident: 10.1016/j.ijmm.2015.08.001_bib0115 article-title: Identification and characterization of a new conjugation/type IVA secretion system (trb/tra) of Legionella pneumophila Corby localized on two mobile genomic islands publication-title: Int. J. Med. Microbiol. doi: 10.1016/j.ijmm.2007.07.012 contributor: fullname: Glöckner – volume: 5 year: 2014 ident: 10.1016/j.ijmm.2015.08.001_bib0095 article-title: Integrative conjugative element ICE-betaox confers oxidative stress resistance to Legionella pneumophila in vitro and in macrophages publication-title: MBio doi: 10.1128/mBio.01091-14 contributor: fullname: Flynn – volume: 65 start-page: 598 year: 2015 ident: 10.1016/j.ijmm.2015.08.001_bib0230 article-title: Legionella norrlandica sp. nov., isolated from the biopurification systems of wood processing plants publication-title: Int. J. Syst. Evol. Microbiol. doi: 10.1099/ijs.0.068940-0 contributor: fullname: Rizzardi – volume: 15 start-page: 504 year: 2014 ident: 10.1016/j.ijmm.2015.08.001_bib0205 article-title: Gene flow in environmental Legionella pneumophila leads to genetic and pathogenic heterogeneity within a Legionnaires’ disease outbreak publication-title: Genome Biol. contributor: fullname: McAdam – volume: 35 start-page: 118 year: 2003 ident: 10.1016/j.ijmm.2015.08.001_bib0220 article-title: Primer design for automated DNA sequencing in a core facility publication-title: Biotechniques contributor: fullname: O'Shaughnessy – volume: 315 start-page: 1709 year: 2007 ident: 10.1016/j.ijmm.2015.08.001_bib0015 article-title: CRISPR provides acquired resistance against viruses in prokaryotes publication-title: Science doi: 10.1126/science.1138140 contributor: fullname: Barrangou – volume: 71 start-page: 282 year: 2005 ident: 10.1016/j.ijmm.2015.08.001_bib0010 article-title: Clinical and environmental isolates of Legionella pneumophila serogroup 1 cannot be distinguished by sequence analysis of two surface protein genes and three housekeeping genes publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.71.1.282-289.2005 contributor: fullname: Aurell – volume: 9 start-page: e103852 year: 2014 ident: 10.1016/j.ijmm.2015.08.001_bib0185 article-title: Genomic characterization of a large outbreak of Legionella pneumophila serogroup 1 strains in Quebec City, 2012 publication-title: PLOS ONE doi: 10.1371/journal.pone.0103852 contributor: fullname: Levesque – volume: 90 start-page: 81 year: 1983 ident: 10.1016/j.ijmm.2015.08.001_bib0085 article-title: Aerosol infection of animals with strains of Legionella pneumophila of different virulence: comparison with intraperitoneal and intranasal routes of infection publication-title: J. Hyg. (Lond.) doi: 10.1017/S0022172400063877 contributor: fullname: Fitzgeorge – volume: 20 start-page: 121S year: 1991 ident: 10.1016/j.ijmm.2015.08.001_bib0180 article-title: Survival and growth of Legionella species in the environment publication-title: Soc. Appl. Bacteriol. Symp. Ser. contributor: fullname: Lee – volume: 195 start-page: 1583 year: 2013 ident: 10.1016/j.ijmm.2015.08.001_bib0165 article-title: Regulation, integrase-dependent excision, and horizontal transfer of genomic islands in Legionella pneumophila publication-title: J. Bacteriol. doi: 10.1128/JB.01739-12 contributor: fullname: Lautner – volume: 186 start-page: 127 year: 2002 ident: 10.1016/j.ijmm.2015.08.001_bib0290 article-title: Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey publication-title: J. Infect. Dis. doi: 10.1086/341087 contributor: fullname: Yu – volume: 297 start-page: 1189 year: 1977 ident: 10.1016/j.ijmm.2015.08.001_bib0100 article-title: Legionnaires’ disease: description of an epidemic of pneumonia publication-title: N. Engl. J. Med. doi: 10.1056/NEJM197712012972201 contributor: fullname: Fraser – volume: 11 start-page: 181 year: 2010 ident: 10.1016/j.ijmm.2015.08.001_bib0055 article-title: Legionella pneumophila pangenome reveals strain-specific virulence factors publication-title: BMC Genomics doi: 10.1186/1471-2164-11-181 contributor: fullname: D’Auria – volume: 152 start-page: 3561 year: 2006 ident: 10.1016/j.ijmm.2015.08.001_bib0065 article-title: Growth-phase-dependent mobility of the lvh-encoding region in Legionella pneumophila strain Paris publication-title: Microbiology doi: 10.1099/mic.0.29227-0 contributor: fullname: Doleans-Jordheim – volume: 83 start-page: 1008 year: 2015 ident: 10.1016/j.ijmm.2015.08.001_bib0140 article-title: Nuclease activity of Legionella pneumophila Cas2 promotes intracellular infection of amoebal host cells publication-title: Infect. Immun. doi: 10.1128/IAI.03102-14 contributor: fullname: Gunderson – volume: 15 start-page: 19472 year: 2010 ident: 10.1016/j.ijmm.2015.08.001_bib0265 article-title: Preliminary report: outbreak of Legionnaires disease in the cities of Ulm and Neu-Ulm in Germany December 2009–January 2010 publication-title: Eur. Surveill. doi: 10.2807/ese.15.04.19472-en contributor: fullname: von Baum – volume: 4 year: 2013 ident: 10.1016/j.ijmm.2015.08.001_bib0135 article-title: The CRISPR-associated gene cas2 of Legionella pneumophila is required for intracellular infection of amoebae publication-title: MBio doi: 10.1128/mBio.00074-13 contributor: fullname: Gunderson – volume: 6 start-page: 181 year: 2008 ident: 10.1016/j.ijmm.2015.08.001_bib0260 article-title: CRISPR – a widespread system that provides acquired resistance against phages in bacteria and archaea publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro1793 contributor: fullname: Sorek – volume: 36 start-page: 1165 year: 2004 ident: 10.1016/j.ijmm.2015.08.001_bib0045 article-title: Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity publication-title: Nat. Genet. doi: 10.1038/ng1447 contributor: fullname: Cazalet – start-page: 3 year: 2015 ident: 10.1016/j.ijmm.2015.08.001_bib0270 article-title: Draft genome sequences of five Legionella pneumophila strains isolated from environmental water samples publication-title: Genome Announc. contributor: fullname: Watanabe – volume: 14 start-page: 298 year: 1981 ident: 10.1016/j.ijmm.2015.08.001_bib0070 article-title: Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.14.3.298-303.1981 contributor: fullname: Edelstein – volume: 90 start-page: 656 year: 1979 ident: 10.1016/j.ijmm.2015.08.001_bib0020 article-title: Classification of the Legionnaires’ disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova publication-title: Ann. Intern. Med. doi: 10.7326/0003-4819-90-4-656 contributor: fullname: Brenner – volume: 21 start-page: 710 year: 2002 ident: 10.1016/j.ijmm.2015.08.001_bib0150 article-title: Pan-European study on culture-proven Legionnaires’ disease: distribution of Legionella pneumophila serogroups and monoclonal subgroups publication-title: Eur. J. Clin. Microbiol. Infect. Dis. doi: 10.1007/s10096-002-0820-3 contributor: fullname: Helbig – volume: 13 start-page: 198 year: 2013 ident: 10.1016/j.ijmm.2015.08.001_bib0225 article-title: A structural comparison of lipopolysaccharide biosynthesis loci of Legionella pneumophila serogroup 1 strains publication-title: BMC Microbiol. doi: 10.1186/1471-2180-13-198 contributor: fullname: Petzold – volume: 95 start-page: 29 year: 1985 ident: 10.1016/j.ijmm.2015.08.001_bib0155 article-title: A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs publication-title: J. Hyg. (Lond.) doi: 10.1017/S0022172400062252 contributor: fullname: Jepras – volume: 35 start-page: 907 year: 1997 ident: 10.1016/j.ijmm.2015.08.001_bib0160 article-title: Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.35.4.907-914.1997 contributor: fullname: Kamerbeek – volume: 37 start-page: 2652 year: 1999 ident: 10.1016/j.ijmm.2015.08.001_bib0170 article-title: Single clonal origin of a high proportion of Legionella pneumophila serogroup 1 isolates from patients and the environment in the area of Paris, France, over a 10-year period publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.37.8.2652-2655.1999 contributor: fullname: Lawrence – volume: 8 start-page: e82221 year: 2013 ident: 10.1016/j.ijmm.2015.08.001_bib0275 article-title: A distinct and divergent lineage of genomic island-associated type IV secretion systems in Legionella publication-title: PLOS ONE doi: 10.1371/journal.pone.0082221 contributor: fullname: Wee – volume: 4 start-page: 286 year: 1996 ident: 10.1016/j.ijmm.2015.08.001_bib0075 article-title: The molecular ecology of legionellae publication-title: Trends Microbiol. doi: 10.1016/0966-842X(96)10041-X contributor: fullname: Fields – volume: 50 start-page: 696 year: 2012 ident: 10.1016/j.ijmm.2015.08.001_bib0110 article-title: Legionella pneumophila sequence type 1/Paris pulsotype subtyping by spoligotyping publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.06180-11 contributor: fullname: Ginevra – volume: 90 start-page: 451 year: 1983 ident: 10.1016/j.ijmm.2015.08.001_bib0145 article-title: Survival of virulent Legionella pneumophila in aerosols publication-title: J. Hyg. (Lond.) doi: 10.1017/S0022172400029090 contributor: fullname: Hambleton – volume: 14 start-page: 673 year: 2008 ident: 10.1016/j.ijmm.2015.08.001_bib0105 article-title: Lorraine strain of Legionella pneumophila serogroup 1 publication-title: France. Emerg. Infect. Dis. doi: 10.3201/eid1404.070961 contributor: fullname: Ginevra – volume: 482 start-page: 331 year: 2012 ident: 10.1016/j.ijmm.2015.08.001_bib0280 article-title: RNA-guided genetic silencing systems in bacteria and archaea publication-title: Nature doi: 10.1038/nature10886 contributor: fullname: Wiedenheft – volume: 30 start-page: 2068 year: 2014 ident: 10.1016/j.ijmm.2015.08.001_bib0250 article-title: Prokka: rapid prokaryotic genome annotation publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu153 contributor: fullname: Seemann – volume: 18 start-page: 431 year: 2008 ident: 10.1016/j.ijmm.2015.08.001_bib0040 article-title: Multigenome analysis identifies a worldwide distributed epidemic Legionella pneumophila clone that emerged within a highly diverse species publication-title: Genome Res. doi: 10.1101/gr.7229808 contributor: fullname: Cazalet – volume: 65 start-page: 135 year: 1988 ident: 10.1016/j.ijmm.2015.08.001_bib0060 article-title: Differences in aerosol survival between pathogenic and non-pathogenic strains of Legionella pneumophila serogroup 1 publication-title: J. Appl. Bacteriol. doi: 10.1111/j.1365-2672.1988.tb01501.x contributor: fullname: Dennis – volume: 25 start-page: 2271 year: 2009 ident: 10.1016/j.ijmm.2015.08.001_bib0200 article-title: IMG ER: a system for microbial genome annotation expert review and curation publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp393 contributor: fullname: Markowitz – volume: 22 start-page: 678 year: 1986 ident: 10.1016/j.ijmm.2015.08.001_bib0240 article-title: Current views on the relationships between amoebae, legionellae and man publication-title: Isr. J. Med. Sci. contributor: fullname: Rowbotham – volume: 28 start-page: 95 year: 2015 ident: 10.1016/j.ijmm.2015.08.001_bib0210 article-title: Current and emerging Legionella diagnostics for laboratory and outbreak investigations publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.00029-14 contributor: fullname: Mercante – volume: 27 start-page: 1 year: 1980 ident: 10.1016/j.ijmm.2015.08.001_bib0255 article-title: Legionnaires’ disease publication-title: Dis. Mon. doi: 10.1016/S0011-5029(80)80010-1 contributor: fullname: Shands |
SSID | ssj0002936 |
Score | 2.226171 |
Snippet | Abstract In 2009/2010 an outbreak of Legionnaires’ disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L.... In 2009/2010 an outbreak of Legionnaires’ disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila... In 2009/2010 an outbreak of Legionnaires' disease with 64 cases including four fatalities took place in the city of Ulm/Neu-Ulm in Germany. L. pneumophila... |
SourceID | proquest crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 828 |
SubjectTerms | CRISPR-Cas Systems CRISPR–Cas Disease Outbreaks Environmental Microbiology Genetic Variation Genomic island Genomic Islands Genotype Germany - epidemiology Humans Infectious Disease Legionella pneumophila - classification Legionella pneumophila - genetics Legionella pneumophila - isolation & purification Legionella pneumophila Ulm Legionnaires' Disease - epidemiology Legionnaires' Disease - microbiology Medical Education Molecular Epidemiology Outbreak |
Title | Subtyping of the Legionella pneumophila “Ulm” outbreak strain using the CRISPR–Cas system |
URI | https://www.clinicalkey.es/playcontent/1-s2.0-S1438422115000624 https://dx.doi.org/10.1016/j.ijmm.2015.08.001 https://www.ncbi.nlm.nih.gov/pubmed/26294350 https://search.proquest.com/docview/1750425415 |
Volume | 305 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB71ISQuFVAeC2VlJG5V2NiJE3KsIqottFW17Uq9WY7XKVvYh5rsoRe0_4Er_Ln9JZ2JnZUqoAduSRTLydgz84083wzA-8zoLC2NCMLiYxLEIoyDgicSgZzWNpPWuN6AJ6dJfxh_vpSXG5C3XBhKq_S239n0xlr7Jz0vzd58PO6dU-PuWAiCNMQEjDdhG90RndVuHxx96Z-uDTJ6NEcyQt2mAZ4749K8xtcTIqRz2VTy9L1h_uKf_oU_Gz90-AR2PIBkB-4bn8KGnT6DR66l5O0uKLQE9S2RoNisZIju2LGllGNKcmLzqV1MZvOvY7xeLX8Nv09Wy99stqgxLtbfWNX0i2CUCn_VDM0HR-dng9XyZ64r5mo-P4fh4aeLvB_4JgqBQTBUB6PCoKMajXSaxJnhVmRJWZaIOiTqcmgSDEQjzXWGb5M66jDSAmGKiRDnUWwYvYCtKX7lK2BFYVLNpS2tHCGOS3URZYWx6PFLLeIo7MB-Kzo1d7UyVJtEdq1I0IoErajvZcg7kLbSVS0LFO2WrbwSVYqrSqhQ_bHQHZDrkff2ikI38OCM79pFVKhEdDKip3a2wJmoyD2Gylx24KVb3fUfiERkiCnD1_856xt4THcuBWYPtuqbhX2LQKYuurD54Qfv4nbNB8dnXb9t7wCaI_Pf |
link.rule.ids | 314,780,784,4502,24116,27924,27925,45585,45679 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NTttAEB5RUFUuiJ8CgQJbqTdkYq-9dn2sIqIAASEgErfVerOG0OZH2DnkgvIOvdKXy5N0xmuDUCkHbpa9q7Vnd2a-kb-ZAfgWaxVHqeaOm3wPnYC7gZN4oUAgp5SJhdG2N-DpWdjqBMfX4noOGlUuDNEqS9tvbXphrcs79VKa9VGvV7-kxt0B5wRpKBMw-AALgUD0i4f64OGZ54H-zKYYoWbT8DJzxpK8end9Skf3RFHHs-wM84p3-h_6LLxQcxmWSvjIftg3XIE5M1iFj7ah5GQNJNqBfEIpUGyYMsR2rG2IcEwUJzYamHF_OLrt4fVs-tj51Z9N_7DhOMeoWP1kWdEtghER_qaY2rg4ujy_mE1_N1TGbMXnz9BpHl41Wk7ZQsHRCIVyp5todFPdrorCINae4XGYpiliDoGa7OoQw1BfeSrG0aSMyvUVR5CifUR5FBn66zA_wLfcBJYkOlKeMKkRXURxkUr8ONEG_X2qeOC7NdivRCdHtlKGrChkd5IELUnQkrpeul4Nokq6ssoBRatlslKFMunJjEtX_rPNNRBPM1-cFIlO4M0Vv1abKFGF6L-IGpjhGFeiEvcYKHuiBht2d5--gIc8RkTpbr1z1T341Lo6bcv20dnJNizSE0uG-QLz-f3Y7CCkyZPd4sj-BTUw8yM |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Subtyping+of+the+Legionella+pneumophila+%22Ulm%22+outbreak+strain+using+the+CRISPR-Cas+system&rft.jtitle=International+journal+of+medical+microbiology&rft.au=L%C3%BCck%2C+Christian&rft.au=Brzuszkiewicz%2C+Elzbieta&rft.au=Rydzewski%2C+Kerstin&rft.au=Koshkolda%2C+Tetyana&rft.date=2015-12-01&rft.eissn=1618-0607&rft.volume=305&rft.issue=8&rft.spage=828&rft.epage=837&rft_id=info:doi/10.1016%2Fj.ijmm.2015.08.001&rft.externalDBID=NO_FULL_TEXT |
thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=https%3A%2F%2Fcdn.clinicalkey.com%2Fck-thumbnails%2F14384221%2FS1438422115X00087%2Fcov150h.gif |