Determination of bacterial cell volume with the Coulter Counter

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Published inJournal of Bacteriology Vol. 168; no. 3; pp. 1466 - 1467
Main Authors KUBITSCHEK, H. E, FRISKE, J. A
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.12.1986
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AbstractList Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic measurement with a Coulter Counter-Analyzer system and biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.
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Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., (i) electronic measurement with a Coulter Counter-Analyzer system and (ii) biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.
Author H E Kubitschek
J A Friske
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Cites_doi 10.1016/S0006-3495(74)70003-0
10.1073/pnas.57.6.1611
10.1016/S0021-9258(17)41044-1
10.1128/JB.168.2.613-618.1986
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Issue 3
Keywords Measurement
Growing phase
Coulter counter
Escherichia coli
Bacteria
Biophysics
Method
Mean value
Cell volume
Escherichieae
Enterobacteriaceae
Stationary phase
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References Kubitschek H. E. (p_3) 1967; 57
Stock J. B. (p_6) 1977; 252
p_4
Kubitschek H. E. (p_2) 1986; 168
Kubitschek H. E. (p_1) 1974; 14
p_5
References_xml – volume: 14
  start-page: 119
  year: 1974
  ident: p_1
  article-title: Constancy of the ratio of DNA to cell volume in steady-state cultures of Escherichia coli B/r
  publication-title: Biophys. J.
  doi: 10.1016/S0006-3495(74)70003-0
  contributor:
    fullname: Kubitschek H. E.
– volume: 57
  start-page: 1611
  year: 1967
  ident: p_3
  article-title: Onset of DNA .synthesis during the cell cycle in chemostat cultures
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  doi: 10.1073/pnas.57.6.1611
  contributor:
    fullname: Kubitschek H. E.
– ident: p_4
– volume: 252
  start-page: 7850
  year: 1977
  ident: p_6
  article-title: Periplasmic space in Salmonella typhimurium and Escherichia coli
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(17)41044-1
  contributor:
    fullname: Stock J. B.
– ident: p_5
– volume: 168
  start-page: 613
  year: 1986
  ident: p_2
  article-title: Increase in cell mass during the division cycle of Escherichia coli B/rA
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.168.2.613-618.1986
  contributor:
    fullname: Kubitschek H. E.
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Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic...
Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., (i) electronic...
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SubjectTerms Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques - instrumentation
Bacteriology
Biological and medical sciences
Biophysical Phenomena
Biophysics
Cell Count - instrumentation
Escherichia coli
Escherichia coli - ultrastructure
Fundamental and applied biological sciences. Psychology
Microbiology
Title Determination of bacterial cell volume with the Coulter Counter
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