Defining three dimensional chromatin structures of pediatric and adolescent B cells using primary B cell and EBV-immortalized B cell reference genomes

• Published in: BMC medical genomics Vol. 18; no. 1; pp. 97 - 8
• Main Authors: Jiang, Kaiyu, Fu, Yao, Kelly, Jennifer A., Gaffney, Patrick M., Holmes, Lucy C., Jarvis, James N.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28.05.2025; BioMed Central; BMC
• Subjects: Adolescent; Arthritis; Arthritis, Juvenile - genetics; Autoimmune diseases; B cells; B-Lymphocytes - metabolism; Beta cells; CCCTC-Binding Factor - metabolism; Cell lines; Child; Chromatin; Chromatin - chemistry; Chromatin - genetics; Chromatin accessibility; Chromatin conformation; Datasets; Diabetes mellitus (insulin dependent); Diabetes Mellitus, Type 1 - genetics; DNA methylation; Epstein-Barr virus; Female; Genes; Genetic aspects; Genetic research; Genome, Human; Genomes; Genomics; Haplotypes; Health aspects; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - physiology; Human subjects; Humans; Immune system; Juvenile arthritis; Lupus Erythematosus, Systemic - genetics; Pediatric autoimmune disease; Pediatrics; Physiological aspects; Systemic lupus; Systemic lupus erythematosus; Teenagers; United States; United States > US; Chromatin accessibility; Chromatin conformation; Type 1 diabetes; Juvenile arthritis; Systemic lupus; B cells; Pediatric autoimmune disease

Knowledge of the 3D genome is essential to elucidate genetic mechanisms driving autoimmune diseases. The 3D genome is distinct for each cell type, and it is uncertain whether cell lines faithfully recapitulate the 3D architecture of primary human cells or whether developmental aspects of the pediatr...