New Screening Software Shows that Most Recent Large 16S rRNA Gene Clone Libraries Contain Chimeras

• Published in: Applied and Environmental Microbiology Vol. 72; no. 9; pp. 5734 - 5741
• Main Authors: Ashelford, Kevin E, Chuzhanova, Nadia A, Fry, John C, Jones, Antonia J, Weightman, Andrew J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.09.2006
• Subjects: Archaea; Archaea - classification; Archaea - genetics; Bacteria; Bacteria - classification; Bacteria - genetics; Base Sequence; Biological and medical sciences; Chimera; Chimera - genetics; chimerism; classification; clones; Cloning; Cloning, Molecular; computer software; computers; DNA libraries; DNA, Archaeal; DNA, Archaeal - genetics; DNA, Bacterial; DNA, Bacterial - genetics; DNA, Ribosomal; DNA, Ribosomal - genetics; Environmental Microbiology; Fundamental and applied biological sciences. Psychology; Gene Library; Genes; genetics; Internet; Java; languages; Marine Biology; Microbiology; Molecular Sequence Data; nucleotide sequences; Object repository; Operating systems; phylogeny; researchers; ribosomal RNA; RNA, Archaeal; RNA, Archaeal - genetics; RNA, Bacterial; RNA, Bacterial - genetics; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - genetics; screening; Software; 16S-RNA; Screening; Software; Gene library; Ribosomal DNA

A new computer program, called Mallard, is presented for screening entire 16S rRNA gene libraries of up to 1,000 sequences for chimeras and other artifacts. Written in the Java computer language and capable of running on all major operating systems, the program provides a novel graphical approach fo...