H3K4 demethylase SsJMJ11 negatively regulates drought-tolerance responses in sugarcane

Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and pa...

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Published inBMC plant biology Vol. 25; no. 1; pp. 814 - 14
Main Authors Yu, Guangrun, Wu, Xiaoge, Ye, Meiling, Fang, Yuan, Wang, Qiongli
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 02.07.2025
BioMed Central
BMC
Subjects
Abscisic acid
Abscisic Acid - metabolism
Adaptation
Arabidopsis - genetics
Botanical research
China
Dehydration
Drought
Drought resistance
Drought stress
Droughts
Environmental aspects
Flowering
Gene Expression Regulation, Plant
Gene loci
Genetic aspects
Genomes
Genomics
H3K4me3
Hardiness
Histone demethylase
Histone Demethylases - genetics
Histone Demethylases - metabolism
Histones
Histones - metabolism
Homeostasis
Hypersensitivity
JmjC protein
Leaves
Mannitol
Methylation
Methyltransferases
Physiological aspects
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
Plants, Genetically Modified
Plasmids
Proteins
Protoplasts
Saccharum - enzymology
Saccharum - genetics
Saccharum - physiology
Saccharum spontaneum
Soil stresses
Stress, Physiological
Sugarcane
China
Drought stress
Histone demethylase
JmjC protein
H3K4me3
Saccharum spontaneum
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Abstract Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive. We show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci. Our results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.
AbstractList Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive. We show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci. Our results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.
Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive.BACKGROUNDDrought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive.We show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci.RESULTSWe show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci.Our results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.CONCLUSIONSOur results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.
Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive. We show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci. Our results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.
Abstract Background Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive. Results We show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci. Conclusions Our results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.
BackgroundDrought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive.ResultsWe show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci.ConclusionsOur results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane.
Background Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated activity of methyltransferases and demethylases. We previously demonstrated that SsJMJ11 is an H3K4me3 demethylase in Saccharum spontaneum and participates in regulating flowering time. However, the role of H3K4me3 regulators in regulating drought-stress responses in sugarcane (Saccharum spp.) remains elusive. Results We show that SsJMJ11 negatively regulates drought-stress responses by acting as an H3K4me3 demethylase. Ectopic overexpression of SsJMJ11 in Arabidopsis thaliana resulted in a hypersensitivity to soil drought stress as well as abscisic acid (ABA) and mannitol. Meanwhile, the drought-induced expression of AtRD20 and AtDREB2A, two well-known positive regulators of drought tolerance, was repressed by SsJMJ11 overexpression. In S. spontaneum, the ABA- and dehydration-induced transcription of SsRD20 and SsDREB2A was associated with increased H3K4me3 levels at these loci. Furthermore, transient overexpression of SsJMJ11 in S. spontaneum protoplasts reduced the ABA-induced transcription of SsRD20 and SsDREB2A, paralleling reduced H3K4me3 levels at these loci. Conclusions Our results suggest that SsJMJ11-mediated dynamic deposition of H3K4me3 is required for proper adaptation to drought stress in sugarcane. Keywords: Drought stress, Histone demethylase, JmjC protein, H3K4me3, Saccharum spontaneum
ArticleNumber 814
Audience Academic
Author Wang, Qiongli
Wu, Xiaoge
Yu, Guangrun
Fang, Yuan
Ye, Meiling
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Cites_doi 10.1038/nsmb1216
10.1105/tpc.105.035881
10.1016/j.plantsci.2023.111920
10.1038/nature16467
10.1111/nph.17177
10.1111/jipb.13474
10.1007/s12355-023-01352-2
10.1016/j.plaphy.2018.09.007
10.1093/plphys/kiab020
10.1111/j.1744-7909.2008.00692.x
10.1016/j.cell.2007.02.017
10.1073/pnas.190309197
10.1371/journal.pgen.1002664
10.1016/0092-8674(91)90436-3
10.1093/jxb/erae037
10.1038/cr.2010.27
10.1126/science.adf8822
10.1093/plphys/kiae461
10.1038/s41477-025-01924-y
10.1093/pcp/pcs053
10.1186/1471-2229-10-238
10.1093/nar/gku1382
10.1016/j.pbi.2022.102211
10.1016/j.cj.2020.05.006
10.1046/j.0960-7412.2001.01227.x
10.1146/annurev.arplant.043008.091939
10.1074/jbc.275.3.1723
10.1105/tpc.104.025353
10.1038/s41588-018-0237-2
10.1016/j.pbi.2016.08.002
10.1111/nph.15874
10.1046/j.1365-313X.1994.6020259.x
10.1093/pcp/pcq155
10.1073/pnas.0605639103
10.1111/nph.17593
10.1007/s11427-024-2784-3
10.1105/tpc.18.00693
10.1038/s41467-021-23766-w
10.1038/nrm2143
10.1038/s41422-019-0145-8
10.1186/s13059-015-0721-2
10.1016/j.tibs.2023.06.001
10.1371/journal.pgen.1003239
10.1038/nature13722
10.1002/bies.201600095
10.1111/jipb.12654
10.1007/s11427-023-2361-1
10.1105/tpc.112.103119
10.1104/pp.125.2.935
10.1093/plphys/kiac095
10.1016/j.indcrop.2024.118718
10.1007/978-981-13-1244-1_12
10.1038/nrg3901
10.1105/tpc.010362
10.1105/tpc.113.113886
10.1073/pnas.0903144106
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Issue 1
Keywords Drought stress
Histone demethylase
JmjC protein
H3K4me3
Saccharum spontaneum
Language English
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References P Liu (6832_CR23) 2019; 31
Q Wang (6832_CR26) 2021; 232
S Iwase (6832_CR16) 2007; 128
DQ Chen (6832_CR29) 2024; 216
G Yu (6832_CR28) 2024; 75
C Liu (6832_CR14) 2010; 61
JZ Liu (6832_CR57) 2019; 29
Y Xue (6832_CR7) 2025
Q Wang (6832_CR30) 2021; 9
TJ Wang (6832_CR45) 2021; 230
M Wang (6832_CR56) 2025; 11
ZL Tian (6832_CR54) 2024; 338
H Hu (6832_CR11) 2022; 67
F Huang (6832_CR13) 2023; 381
P Crevill (6832_CR53) 2014; 515
T Taji (6832_CR42) 2002; 29
DM Emms (6832_CR32) 2015; 16
H Fujii (6832_CR35) 2009; 106
Z Shi (6832_CR44) 2023; 66
RJ Klose (6832_CR17) 2007; 8
Q Chen (6832_CR22) 2013; 9
J Zhang (6832_CR3) 2018; 50
QN Yong (6832_CR50) 2015; 43
N Yamaguchi (6832_CR6) 2021; 12
S Huang (6832_CR25) 2019; 223
S Agurla (6832_CR33) 2018; 1081
MV Mickelbart (6832_CR5) 2015; 16
I Le Masson (6832_CR20) 2012; 24
PJ Franks (6832_CR31) 2001; 125
Y Aubert (6832_CR43) 2010; 51
F Lu (6832_CR12) 2008; 50
J Qi (6832_CR34) 2018; 60
JM Kim (6832_CR10) 2012; 53
JY Kang (6832_CR38) 2002; 14
DR McCarty (6832_CR39) 1991; 66
XT Zhang (6832_CR1) 2025; S1674–2052
N Lee (6832_CR18) 2007; 14
B Noh (6832_CR52) 2004; 16
Y Sakuma (6832_CR48) 2006; 18
FS Howe (6832_CR9) 2017; 39
X Cui (6832_CR24) 2021; 185
T Soderman (6832_CR41) 1994; 35
YL Fu (6832_CR51) 2013; 25
FL Lu (6832_CR19) 2010; 20
WJ Zhao (6832_CR46) 2022; 189
H Yang (6832_CR21) 2012; 8
H Choi (6832_CR36) 2000; 275
J Zhu (6832_CR58) 2023; 48
C Lesk (6832_CR2) 2016; 529
Y Uno (6832_CR37) 2000; 97
T Zhao (6832_CR27) 2018; 132
AK Mall (6832_CR4) 2024
T Kiyosue (6832_CR40) 1994; 6
QH Di (6832_CR55) 2025; 197
J Xiao (6832_CR15) 2016; 34
Y Sakuma (6832_CR49) 2006; 103
W Wei (6832_CR47) 2023; 65
DK Van (6832_CR8) 2010; 10
References_xml – volume: 14
  start-page: 341
  year: 2007
  ident: 6832_CR18
  publication-title: Nat Struct Mol Biol
  doi: 10.1038/nsmb1216
– volume: 18
  start-page: 1292
  issue: 5
  year: 2006
  ident: 6832_CR48
  publication-title: Plant Cell
  doi: 10.1105/tpc.105.035881
– volume: 338
  start-page: 111920
  year: 2024
  ident: 6832_CR54
  publication-title: Plant Sci
  doi: 10.1016/j.plantsci.2023.111920
– volume: 529
  start-page: 84
  year: 2016
  ident: 6832_CR2
  publication-title: Nature
  doi: 10.1038/nature16467
– volume: 230
  start-page: 567
  year: 2021
  ident: 6832_CR45
  publication-title: New Phytol
  doi: 10.1111/nph.17177
– volume: 65
  start-page: 1636
  year: 2023
  ident: 6832_CR47
  publication-title: J Integr Plant Biol
  doi: 10.1111/jipb.13474
– year: 2024
  ident: 6832_CR4
  publication-title: Sugar Tech
  doi: 10.1007/s12355-023-01352-2
– volume: 132
  start-page: 183
  year: 2018
  ident: 6832_CR27
  publication-title: Plant Physiol Biochem
  doi: 10.1016/j.plaphy.2018.09.007
– volume: 185
  start-page: 1813
  year: 2021
  ident: 6832_CR24
  publication-title: Plant Physiol
  doi: 10.1093/plphys/kiab020
– volume: 50
  start-page: 886
  year: 2008
  ident: 6832_CR12
  publication-title: J Integr Plant Biol
  doi: 10.1111/j.1744-7909.2008.00692.x
– volume: 128
  start-page: 1077
  year: 2007
  ident: 6832_CR16
  publication-title: Cell
  doi: 10.1016/j.cell.2007.02.017
– volume: 97
  start-page: 11632
  year: 2000
  ident: 6832_CR37
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.190309197
– volume: 8
  start-page: e1002664
  year: 2012
  ident: 6832_CR21
  publication-title: PLoS Genet
  doi: 10.1371/journal.pgen.1002664
– volume: 66
  start-page: 895
  year: 1991
  ident: 6832_CR39
  publication-title: Cell
  doi: 10.1016/0092-8674(91)90436-3
– volume: 75
  start-page: 3040
  year: 2024
  ident: 6832_CR28
  publication-title: J Exp Bot
  doi: 10.1093/jxb/erae037
– volume: 20
  start-page: 387
  year: 2010
  ident: 6832_CR19
  publication-title: Cell Res
  doi: 10.1038/cr.2010.27
– volume: 381
  start-page: eadf8822
  year: 2023
  ident: 6832_CR13
  publication-title: Science
  doi: 10.1126/science.adf8822
– volume: 197
  start-page: kiae461
  issue: 2
  year: 2025
  ident: 6832_CR55
  publication-title: Plant Physiol
  doi: 10.1093/plphys/kiae461
– volume: 11
  start-page: 206
  issue: 2
  year: 2025
  ident: 6832_CR56
  publication-title: Nat Plants
  doi: 10.1038/s41477-025-01924-y
– volume: 53
  start-page: 847
  year: 2012
  ident: 6832_CR10
  publication-title: Plant Cell Physiol
  doi: 10.1093/pcp/pcs053
– volume: 10
  start-page: 238
  year: 2010
  ident: 6832_CR8
  publication-title: BMC Plant Biol
  doi: 10.1186/1471-2229-10-238
– volume: 43
  start-page: 1469
  issue: 3
  year: 2015
  ident: 6832_CR50
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gku1382
– volume: 67
  start-page: 102211
  year: 2022
  ident: 6832_CR11
  publication-title: Curr Opin Plant Biol
  doi: 10.1016/j.pbi.2022.102211
– volume: 9
  start-page: 133
  year: 2021
  ident: 6832_CR30
  publication-title: Crop J
  doi: 10.1016/j.cj.2020.05.006
– volume: 29
  start-page: 417
  year: 2002
  ident: 6832_CR42
  publication-title: Plant J
  doi: 10.1046/j.0960-7412.2001.01227.x
– volume: 61
  start-page: 395
  year: 2010
  ident: 6832_CR14
  publication-title: Annu Rev Plant Biol
  doi: 10.1146/annurev.arplant.043008.091939
– volume: 275
  start-page: 1723
  year: 2000
  ident: 6832_CR36
  publication-title: J Biol Chem
  doi: 10.1074/jbc.275.3.1723
– volume: 16
  start-page: 2601
  year: 2004
  ident: 6832_CR52
  publication-title: Plant Cell
  doi: 10.1105/tpc.104.025353
– volume: 50
  start-page: 1565
  year: 2018
  ident: 6832_CR3
  publication-title: Nat Genet
  doi: 10.1038/s41588-018-0237-2
– volume: 34
  start-page: 41
  year: 2016
  ident: 6832_CR15
  publication-title: Curr Opin Plant Biol
  doi: 10.1016/j.pbi.2016.08.002
– volume: 223
  start-page: 1372
  year: 2019
  ident: 6832_CR25
  publication-title: New Phytol
  doi: 10.1111/nph.15874
– volume: 6
  start-page: 259
  year: 1994
  ident: 6832_CR40
  publication-title: Plant J
  doi: 10.1046/j.1365-313X.1994.6020259.x
– volume: 51
  start-page: 1975
  year: 2010
  ident: 6832_CR43
  publication-title: Plant Cell Physiol
  doi: 10.1093/pcp/pcq155
– volume: S1674–2052
  start-page: 3
  issue: 25
  year: 2025
  ident: 6832_CR1
  publication-title: Mol Plant
– volume: 103
  start-page: 18822
  issue: 49
  year: 2006
  ident: 6832_CR49
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.0605639103
– volume: 232
  start-page: 221
  year: 2021
  ident: 6832_CR26
  publication-title: New Phytol
  doi: 10.1111/nph.17593
– year: 2025
  ident: 6832_CR7
  publication-title: Sci China Life Sci
  doi: 10.1007/s11427-024-2784-3
– volume: 31
  start-page: 430
  year: 2019
  ident: 6832_CR23
  publication-title: Plant Cell
  doi: 10.1105/tpc.18.00693
– volume: 12
  start-page: 3480
  year: 2021
  ident: 6832_CR6
  publication-title: Nat Commun
  doi: 10.1038/s41467-021-23766-w
– volume: 35
  start-page: 225
  year: 1994
  ident: 6832_CR41
  publication-title: Plant Cell Physiol
– volume: 8
  start-page: 307
  year: 2007
  ident: 6832_CR17
  publication-title: Nat Rev Mol Cell Biol
  doi: 10.1038/nrm2143
– volume: 29
  start-page: 379
  issue: 5
  year: 2019
  ident: 6832_CR57
  publication-title: Cell Res
  doi: 10.1038/s41422-019-0145-8
– volume: 16
  start-page: 157
  year: 2015
  ident: 6832_CR32
  publication-title: Genome Biol
  doi: 10.1186/s13059-015-0721-2
– volume: 48
  start-page: 788
  issue: 9
  year: 2023
  ident: 6832_CR58
  publication-title: Trends Biochem Sci
  doi: 10.1016/j.tibs.2023.06.001
– volume: 9
  start-page: e1003239
  year: 2013
  ident: 6832_CR22
  publication-title: PLoS Genet
  doi: 10.1371/journal.pgen.1003239
– volume: 515
  start-page: 587
  year: 2014
  ident: 6832_CR53
  publication-title: Nature
  doi: 10.1038/nature13722
– volume: 39
  start-page: 1
  year: 2017
  ident: 6832_CR9
  publication-title: BioEssays
  doi: 10.1002/bies.201600095
– volume: 60
  start-page: 805
  year: 2018
  ident: 6832_CR34
  publication-title: J Integr Plant Biol
  doi: 10.1111/jipb.12654
– volume: 66
  start-page: 1903
  year: 2023
  ident: 6832_CR44
  publication-title: Sci China Life Sci
  doi: 10.1007/s11427-023-2361-1
– volume: 24
  start-page: 3603
  year: 2012
  ident: 6832_CR20
  publication-title: Plant Cell
  doi: 10.1105/tpc.112.103119
– volume: 125
  start-page: 935
  year: 2001
  ident: 6832_CR31
  publication-title: Plant Physiol
  doi: 10.1104/pp.125.2.935
– volume: 189
  start-page: 1050
  year: 2022
  ident: 6832_CR46
  publication-title: Plant Physiol
  doi: 10.1093/plphys/kiac095
– volume: 216
  start-page: 118718
  year: 2024
  ident: 6832_CR29
  publication-title: Ind Crops Prod
  doi: 10.1016/j.indcrop.2024.118718
– volume: 1081
  start-page: 215
  year: 2018
  ident: 6832_CR33
  publication-title: Adv Exp Med Biol
  doi: 10.1007/978-981-13-1244-1_12
– volume: 16
  start-page: 237
  year: 2015
  ident: 6832_CR5
  publication-title: Nat Rev Genet
  doi: 10.1038/nrg3901
– volume: 14
  start-page: 343
  year: 2002
  ident: 6832_CR38
  publication-title: Plant Cell
  doi: 10.1105/tpc.010362
– volume: 25
  start-page: 2878
  year: 2013
  ident: 6832_CR51
  publication-title: Plant Cell
  doi: 10.1105/tpc.113.113886
– volume: 106
  start-page: 8380
  year: 2009
  ident: 6832_CR35
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.0903144106
SSID ssj0017849
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Snippet Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the coordinated...
Background Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the...
BackgroundDrought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on the...
Abstract Background Drought-induced gene alteration is usually associated with changes of histone H3K4me3 in plants. Histone methylation homeostasis relies on...
SourceID doaj
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SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 814
SubjectTerms Abscisic acid
Abscisic Acid - metabolism
Adaptation
Arabidopsis - genetics
Botanical research
China
Dehydration
Drought
Drought resistance
Drought stress
Droughts
Environmental aspects
Flowering
Gene Expression Regulation, Plant
Gene loci
Genetic aspects
Genomes
Genomics
H3K4me3
Hardiness
Histone demethylase
Histone Demethylases - genetics
Histone Demethylases - metabolism
Histones
Histones - metabolism
Homeostasis
Hypersensitivity
JmjC protein
Leaves
Mannitol
Methylation
Methyltransferases
Physiological aspects
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
Plants, Genetically Modified
Plasmids
Proteins
Protoplasts
Saccharum - enzymology
Saccharum - genetics
Saccharum - physiology
Saccharum spontaneum
Soil stresses
Stress, Physiological
Sugarcane
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Title H3K4 demethylase SsJMJ11 negatively regulates drought-tolerance responses in sugarcane
URI https://www.ncbi.nlm.nih.gov/pubmed/40604414
https://www.proquest.com/docview/3227643697
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https://pubmed.ncbi.nlm.nih.gov/PMC12220489
https://doaj.org/article/38ccc0c37ced4157a223b0c3a59a6622
Volume 25
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