A Certified Urea Reference Material (NMIJ CRM 6006-a) as a Reliable Calibrant for the Elemental Analyses of Amino Acids and Food Samples

We examined the reliability of a certified reference material (CRM) for urea (NMIJ CRM 6006-a) as a calibrant for N, C, and H in elemental analyzers. Only the N content for this CRM is provided as an indicative value. To estimate the C and H contents of the urea CRM, we took into account the purity...

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Bibliographic Details
Published inAnalytical Sciences Vol. 30; no. 4; pp. 471 - 476
Main Authors ITOH, Nobuyasu, YAMAZAKI, Taichi, SATO, Ayako, NUMATA, Masahiko, TAKATSU, Akiko
Format Journal Article
LanguageEnglish
Published Singapore The Japan Society for Analytical Chemistry 2014
Springer Nature Singapore
Japan Science and Technology Agency
Subjects
Amino Acids - analysis
Analytical Chemistry
Animals
calibrant
Calibration
Carbon - analysis
Chemistry
CHN
elemental analyzer
Fish Products - analysis
Food Analysis - instrumentation
Food Analysis - methods
Glycine max - chemistry
Hydrogen - analysis
Milk - chemistry
Nitrogen - analysis
protein
Reference Standards
Reproducibility of Results
Urea
Urea - analysis
CHN
Urea
elemental analyzer
calibrant
protein
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Summary:We examined the reliability of a certified reference material (CRM) for urea (NMIJ CRM 6006-a) as a calibrant for N, C, and H in elemental analyzers. Only the N content for this CRM is provided as an indicative value. To estimate the C and H contents of the urea CRM, we took into account the purity of the urea and the presence of other identified impurities. When we examined the use of various masses of the calibrant (0.2 to 2 mg), we unexpectedly observed low signal intensities for small masses of H and N, but these plateaued at about 2 mg. We therefore analyzed four amino acid CRMs and four food CRMs on a 2-mg scale with the urea CRM as the calibrant. For the amino acid CRMs, the differences in the analytical and theoretical contents (≤0.0026 kg/kg) were acceptable with good repeatability (≤0.0013 kg/kg in standard deviation; n = 4). For food CRMs, comparable repeatabilities to those obtained with amino acid CRMs (≤0.0025 kg/kg in standard deviation; n = 4) were obtained. The urea CRM can therefore be used as a reliable calibrant for C, H, and N in an elemental analyzer.
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.30.471