A new cytochemical method for in situ detection of cholinergic synaptic transmission by staining of Cu2+ incorporated in frog neuromuscular junction during nerve stimulation

• Published in: Biomedical Research Vol. 27; no. 3; pp. 125 - 130
• Main Authors: HIRAI, Keiji, TANAKA, Eiichiro, MOTELICA-HEINO, Ion, KATAYAMA, Yoshifumi, HIGASHI, Hideo, TSUJI, Shigeru
• Format: Journal Article
• Language: English
• Published: Japan Biomedical Research Press 2006; Japan Science and Technology Agency
• Subjects: 3,3'-Diaminobenzidine - pharmacology; Animals; Copper - metabolism; Copper - pharmacology; Cytoplasm - metabolism; Detergents - pharmacology; Ferrocyanides - pharmacology; Hydrogen Peroxide - pharmacology; Immunohistochemistry; Microscopy, Electron; Neuromuscular Junction - pathology; Neurons - metabolism; Nicotinic Antagonists - pharmacology; Octoxynol - pharmacology; Ranidae; Receptors, Nicotinic - metabolism; Synaptic Transmission

A new cytochemical method was devised in order to visualize Cu2+ ions in the synaptic area after their intracellular penetration during nerve stimulation of the frog neuromuscular junction (NMJ). The motor nerves were stimulated in presence of Cu2+. After total blockade of the neuromuscular junction...