In vitro 50 Hz magnetic field long-term exposure: Cytogenetic tests on human lymphoblastoid TK6 cells and validation of the test environment

Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of...

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Published inMethodsX Vol. 7; p. 101071
Main Authors Nguyen, Ha, Ledent, Maryse, Beauvois, Véronique, Anthonissen, Roel, Verschaeve, Luc, Collard, Jean-Francois, Hinsenkamp, Maurice, Feipel, Veronique, Mertens, Birgit
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.2020
Elsevier
Subjects
Background magnetic field
chronic exposure
Comet assay
cytogenetics
Extremely low-frequency magnetic fields
genotoxicity
humans
magnetic fields
Method
Micronucleus test
Shielding system
Background magnetic field
Micronucleus test
Shielding system
In vitro comet assay, in vitro micronucleus test
Comet assay
Extremely low-frequency magnetic fields
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Abstract Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.•Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results.•Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs.•A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels. [Display omitted]
AbstractList Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well. • Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results. • Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs. • A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels. Image, graphical abstract
Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well. • Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results. • Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs. • A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels.
Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.•Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results.•Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs.•A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels.Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.•Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results.•Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs.•A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels.
Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.•Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results.•Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs.•A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels. [Display omitted]
Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.•Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results.•Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs.•A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels.
ArticleNumber 101071
Author Verschaeve, Luc
Hinsenkamp, Maurice
Nguyen, Ha
Feipel, Veronique
Collard, Jean-Francois
Ledent, Maryse
Mertens, Birgit
Beauvois, Véronique
Anthonissen, Roel
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Keywords Background magnetic field
Micronucleus test
Shielding system
In vitro comet assay, in vitro micronucleus test
Comet assay
Extremely low-frequency magnetic fields
Language English
License This is an open access article under the CC BY license.
2020 The Author(s). Published by Elsevier B.V.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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content type line 23
Ha Nguyen and Maryse Ledent contributed equally to this manuscript as first authors.
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Snippet Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive....
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StartPage 101071
SubjectTerms Background magnetic field
chronic exposure
Comet assay
cytogenetics
Extremely low-frequency magnetic fields
genotoxicity
humans
magnetic fields
Method
Micronucleus test
Shielding system
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Title In vitro 50 Hz magnetic field long-term exposure: Cytogenetic tests on human lymphoblastoid TK6 cells and validation of the test environment
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