An apolipoprotein E4 fragment affects matrix metalloproteinase 9, tissue inhibitor of metalloproteinase 1 and cytokine levels in brain cell lines

• Published in: Neuroscience Vol. 210; pp. 21 - 32
• Main Authors: Dafnis, I, Tzinia, A.K, Tsilibary, E.C, Zannis, V.I, Chroni, A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 17.05.2012; Elsevier
• Subjects: Alzheimer disease; Alzheimer Disease - metabolism; Alzheimer's disease; apolipoprotein E; Apolipoprotein E4; Apolipoprotein E4 - metabolism; Apolipoprotein E4 - pharmacology; apolipoprotein E4 fragments; Astrocytes - drug effects; Astrocytes - metabolism; Astrocytoma; Biological and medical sciences; Blotting, Western; Brain; Brain - drug effects; Brain - metabolism; Cell Line, Tumor; Chromium; Cytokines; Cytokines - drug effects; Cytokines - metabolism; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Extracellular levels; Fundamental and applied biological sciences. Psychology; Gelatinase B; Gene expression; Humans; Inflammation; Inflammation - metabolism; Interleukin 1; Interleukin 10; Matrix metalloproteinase; matrix metalloproteinase 9; Matrix Metalloproteinase 9 - drug effects; Matrix Metalloproteinase 9 - metabolism; Medical sciences; Nervous system; Neurodegenerative diseases; neuroinflammation; Neurology; Neurons - drug effects; Neurons - metabolism; Peptide Fragments - metabolism; Peptide Fragments - pharmacology; Polymerase chain reaction; Proteolysis; Real-Time Polymerase Chain Reaction; Risk factors; Tissue inhibitor of metalloproteinase 1; Tissue Inhibitor of Metalloproteinase-1 - drug effects; Tissue Inhibitor of Metalloproteinase-1 - metabolism; Transfection; Tumor necrosis factor- alpha; Vertebrates: nervous system and sense organs; Western blotting; threshold cycles; TNFα; Alzheimer disease; apolipoprotein E4 fragments; HRP; apoE; phenylmethylsulfonyl fluoride; interleukin-10; tissue inhibitor of metalloproteinase 1; interleukin-1β; Aβ; neuroinflammation; FBS; CSF; ethylenediaminetetraacetic acid; horseradish peroxidase; cytokines; TIMP1; Eagle; WT; quantitative real-time polymerase chain reaction; PBS; AD; apoE4-185; apoE4-165; qRT-PCR; apoE4[Δ(186–299)]; β-amyloid peptide; EDTA; IL-1β; apoE4[Δ(166–299)]; MMP9; IL-10; tumor-necrosis factor α; CT; fetal bovine serum; PMSF; matrix metalloproteinase 9; phosphate buffered saline; cerebrospinal fluid; apolipoprotein E; wild type; Minimum Essential Medium; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Nervous system diseases; Enzyme; Central nervous system; Cytokine; Metalloendopeptidases; Neuroinflammation; Gelatinase B; Cerebral disorder; Encephalon; Peptidases; Cell line; Apolipoprotein E; Central nervous system disease; Hydrolases; matrix metalloproteinase 9, neuroinflammation; Degenerative disease; Metalloproteinase

Abstract Apolipoprotein (apo) E4 isoform, a major risk factor for Alzheimer disease (AD), is more susceptible to proteolysis than apoE2 and apoE3 isoforms. ApoE4 fragments have been found in AD patients' brain. In the present study, we examined the effect of full-length apoE4 and apoE4 fragment...