Enabling malic acid production from corn-stover hydrolysate in Lipomyces starkeyi via metabolic engineering and bioprocess optimization

• Published in: Microbial cell factories Vol. 24; no. 1; pp. 117 - 14
• Main Authors: Czajka, Jeffrey J., Dai, Ziyu, Radivojević, Tijana, Kim, Joonhoon, Deng, Shuang, Lemmon, Teresa, Swita, Marie, Burnet, Meagan C, Munoz, Nathalie, Gao, Yuqian, Kim, Young-Mo, Hofstad, Beth, Magnuson, Jon K., Garcia Martin, Hector, Burnum-Johnson, Kristin E., Pomraning, Kyle R.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 21.05.2025; Springer Science + Business Media; BioMed Central; BMC
• Subjects: Amino acids; Aspergillus oryzae - genetics; Bioreactors; Corn; Engineers; Fermentation; Genetic engineering; Genetically modified organisms; Hydrolysis; Lipids; Lipomyces - genetics; Lipomyces - metabolism; Lipomyces starkeyi; Machine learning; Machine learning medium optimization; Malate Dehydrogenase - genetics; Malate Dehydrogenase - metabolism; Malates - metabolism; Malic acid; Malic acid production; Metabolic engineering; Metabolic Engineering - methods; Methods; Oleaginous yeast; Physiological aspects; Pyruvate Carboxylase - genetics; Pyruvate Carboxylase - metabolism; Yeast fungi; Zea mays - chemistry; Zea mays - metabolism; United States; Malic acid production; Machine learning medium optimization; Oleaginous yeast; Lipomyces starkeyi
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-025-02705-0

Lipomyces starkeyi is an oleaginous yeast with a native metabolism well-suited for production of lipids and biofuels from complex lignocellulosic and waste feedstocks. Recent advances in genetic engineering tools have facilitated the development of L. starkeyi into a microbial chassis for biofuel an...