Expression and inducibility of P450 enzymes during liver ontogeny

• Published in: Microscopy research and technique Vol. 39; no. 5; pp. 424 - 435
• Main Authors: Rich, Kim J., Boobis, Alan R.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.12.1997
• Subjects: Animals; Cytochrome P-450 Enzyme System - biosynthesis; Cytochrome P-450 Enzyme System - classification; Cytochrome P-450 Enzyme System - genetics; development; drug metabolism; Embryo, Mammalian - enzymology; Embryonic and Fetal Development; Enzyme Induction; Gene Expression; gene expression/regulation; hepatocytes; human; Humans; Immunohistochemistry; Liver - embryology; Liver - enzymology; Liver - growth & development; Mice; Rabbits; Rats; rodent

Several approaches, including immunoquantification of individual enzymes, profiling of substrate activities by immunoinhibition using highly specific polyclonal and monoclonal antibodies, and the estimation of corresponding mRNAs with nucleic acid probes, have been used to investigate the ontogeny o...