Electrochemical Monitoring of ROS/RNS Homeostasis Within Individual Phagolysosomes Inside Single Macrophages

• Published in: Angewandte Chemie International Edition Vol. 58; no. 23; pp. 7753 - 7756
• Main Authors: Zhang, Xin‐Wei, Oleinick, Alexander, Jiang, Hong, Liao, Quan‐Lan, Qiu, Quan‐Fa, Svir, Irina, Liu, Yan‐Ling, Amatore, Christian, Huang, Wei‐Hua
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 03.06.2019; Wiley-VCH Verlag
• Edition: International ed. in English
• Subjects: Biochemistry; Biochemistry, Molecular Biology; Cellular Biology; Chemical Sciences; Electrochemical analysis; Electrochemistry; Electrodes; Homeostasis; Life Sciences; Macrophages; Monitoring; nanoelectrode amperometry; Nanotechnology; Nanowires; Other; Oxidation; Phagolysosomes; Reaction kinetics; Reactive nitrogen species; Reactive oxygen species; ROS/RNS production; ROS/RNS production; homeostasis; nanoelectrode amperometry; phagolysosomes

The existence of a homeostatic mechanism regulating reactive oxygen/nitrogen species (ROS/RNS) amounts inside phagolysosomes has been invoked to account for the efficiency of this process but could not be unambiguously documented. Now, intracellular electrochemical analysis with platinized nanowire...