Molecular and biochemical characterization of galectin from amphioxus: primitive galectin of chordates participated in the infection processes

• Published in: Glycobiology (Oxford) Vol. 17; no. 7; pp. 774 - 783
• Main Authors: Yu, Yanhong, Yuan, Shaochun, Yu, Yingcai, Huang, Huiqing, Feng, Kaixia, Pan, Minming, Huang, Shengfeng, Dong, Meiling, Chen, Shangwu, Xu, Anlong
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.07.2007; Oxford Publishing Limited (England)
• Subjects: alternative splicing; Amino Acid Sequence; amphioxus; Animals; bacteria; Base Sequence; Carbohydrates - chemistry; Chordata; Chordata - metabolism; Chordata - microbiology; diverticulum; embryogenesis; Embryonic growth stage; Escherichia coli - metabolism; evolution; Evolution, Molecular; exons; galectin; galectins; Galectins - chemistry; Galectins - metabolism; gills; host-pathogen relationships; Immune response; Immune system; infection; intestines; introns; Lactose - chemistry; messenger RNA; Microorganisms; Models, Biological; Models, Genetic; Molecular Sequence Data; Phylogeny; quantitative polymerase chain reaction; recombinant proteins; Recombinant Proteins - chemistry; Sequence Homology, Amino Acid; tissue distribution; tissues; amphioxus; infection; evolution; galectin

A novel F4-carbohydrate recognition domain (CRD)-linker-F3-CRD-type bi-CRD Branchiostoma belcheri tsingtauense galectin (BbtGal)-L together with its alternatively spliced mono-CRD isoform BbtGal-S from amphioxus intestine was encoded by a 9488-bp unique gene with eight exons and seven introns. The r...