mRNA levels of CD31, CD144, CD146 and von Willebrand factor do not serve as surrogate markers for circulating endothelial cells

• Published in: Thrombosis and haemostasis Vol. 104; no. 2; p. 318
• Main Authors: Strijbos, Michiel H, van Krimpen, Brigitte A, Debets, Reno, Kraan, Jaco, Sleijfer, Stefan, Gratama, Jan Willem, Lamers, Cor H J
• Format: Journal Article
• Language: English
• Published: Germany 01.08.2010
• Subjects: Adult; Antigens, CD - genetics; Apoptosis; Biomarkers - blood; Cadherins - genetics; CD146 Antigen - genetics; Cells, Cultured; Endothelial Cells - metabolism; Endothelial Cells - pathology; Feasibility Studies; Female; Flow Cytometry; Humans; Immunomagnetic Separation; Male; Middle Aged; Platelet Endothelial Cell Adhesion Molecule-1 - genetics; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - blood; T-Lymphocytes - metabolism; von Willebrand Factor - genetics

Circulating endothelial cells (CEC) are considered a promising marker to determine the extent of vascular damage. However, currently available and validated CEC enumeration assays are laborious, time consuming and costly, which limits their clinical utility. Here, we evaluated the feasibility of qua...