Cbfβ Is a Novel Modulator against Osteoarthritis by Maintaining Articular Cartilage Homeostasis through TGF-β Signaling

• Published in: Cells (Basel, Switzerland) Vol. 12; no. 7; p. 1064
• Main Authors: Che, Xiangguo, Jin, Xian, Park, Na Rae, Kim, Hee-June, Kyung, Hee-Soo, Kim, Hyun-Ju, Lian, Jane B, Stein, Janet L, Stein, Gary S, Choi, Je-Yong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.03.2023; MDPI
• Subjects: Animals; Antibodies; Arthritis; articular cartilage; Care and treatment; Cartilage; Cartilage (articular); Cartilage diseases; Cartilage, Articular - metabolism; Chondrocytes; Chondrogenesis; Collagen; Collagen (type II); Core Binding Factor Alpha 2 Subunit - metabolism; Core Binding Factor beta Subunit - metabolism; Degeneration; Extracellular matrix; Health aspects; Homeostasis; Humans; Kinases; Knee; Mice; Osteoarthritis; Osteoarthritis - metabolism; Osteoblastogenesis; Potassium; proteasomal degradation; Proteasomes; Proteins; Runx1 protein; Runx1/Cbfβ complex; Signal Transduction; Smad3 protein; Surgery; TGF-β signaling; Transcription factors; Transforming Growth Factor beta1 - metabolism; Transforming growth factor-b; Transforming growth factor-b1; Transforming growth factors; South Korea; United States > US; articular cartilage; Runx1/Cbfβ complex; proteasomal degradation; TGF-β signaling; osteoarthritis
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12071064

TGF-β signaling is a vital regulator for maintaining articular cartilage homeostasis. Runx transcription factors, downstream targets of TGF-β signaling, have been studied in the context of osteoarthritis (OA). Although Runx partner core binding factor β (Cbfβ) is known to play a pivotal role in chondrocyte and osteoblast differentiation, the role of Cbfβ in maintaining articular cartilage integrity remains obscure. This study investigated Cbfβ as a novel anabolic modulator of TGF-β signaling and determined its role in articular cartilage homeostasis. Cbfβ significantly decreased in aged mouse articular cartilage and human OA cartilage. Articular chondrocyte-specific -deficient mice ( ) exhibited early cartilage degeneration at 20 weeks of age and developed OA at 12 months. mice showed enhanced OA progression under the surgically induced OA model in mice. Mechanistically, forced expression of Cbfβ rescued Type II collagen (Col2α1) and Runx1 expression in Cbfβ-deficient chondrocytes. TGF-β1-mediated Col2α1 expression failed despite the p-Smad3 activation under TGF-β1 treatment in Cbfβ-deficient chondrocytes. Cbfβ protected Runx1 from proteasomal degradation through Cbfβ/Runx1 complex formation. These results indicate that Cbfβ is a novel anabolic regulator for cartilage homeostasis, suggesting that Cbfβ could protect OA development by maintaining the integrity of the TGF-β signaling pathway in articular cartilage.