Identification, expansion and characterization of cancer cells with stem cell properties from head and neck squamous cell carcinomas

• Published in: Experimental cell research Vol. 348; no. 1; pp. 75 - 86
• Main Authors: Kaseb, Hatem O., Fohrer-Ting, Helene, Lewis, Dale W., Lagasse, Eric, Gollin, Susanne M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.10.2016; Elsevier BV; Elsevier
• Subjects: 60 APPLIED LIFE SCIENCES; Adaptive immunology; Aged; Aged, 80 and over; Animals; Antigens, CD - metabolism; BIOLOGICAL MARKERS; Biomarkers, Tumor - metabolism; Cancer; Cancer stem cells; Carcinogenesis - pathology; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; CARCINOMAS; CELL CULTURES; Cell Line, Tumor; Cell Proliferation; Cell Self Renewal; Cell Separation; Cell surface markers; Cellular biology; Chromosomal Instability; CHROMOSOMES; Clone Cells; COMPARATIVE EVALUATIONS; DILUTION; EFFICIENCY; Feeder Cells - cytology; Female; FLUORESCENCE; Fluorescent Antibody Technique; HEAD; Head & neck cancer; Head and Neck Neoplasms - metabolism; Head and Neck Neoplasms - pathology; Head and neck squamous cell carcinoma; Humans; Immunology; In Situ Hybridization, Fluorescence; IN VITRO; IN VIVO; Innate immunity; INSTABILITY; IONIZING RADIATIONS; IRRADIATION; Life Sciences; LUNGS; Male; MICE; Middle Aged; NECK; Neoplastic Stem Cells - metabolism; Neoplastic Stem Cells - pathology; PATIENTS; PUBLIC HEALTH; Radiation Tolerance; Radioresistance; RADIOSENSITIVITY; SOLIDS; SPHEROIDS; Squamous Cell Carcinoma of Head and Neck; STEM CELLS; TUMOR CELLS; CFE; ELDA; NSCLC; OS; Cell surface markers; CIN; IR; Radioresistance; Chromosomal instability; SC; CSC; HNSCC; FISH; Head and neck squamous cell carcinoma; Cancer stem cells; SP

Head and neck squamous cell carcinoma (HNSCC) is a major public health concern. Recent data indicate the presence of cancer stem cells (CSC) in many solid tumors, including HNSCC. Here, we assessed the stem cell (SC) characteristics, including cell surface markers, radioresistance, chromosomal insta...