Conformational Sampling of Aminoacyl-tRNA during Selection on the Bacterial Ribosome

• Published in: Journal of molecular biology Vol. 399; no. 4; pp. 576 - 595
• Main Authors: Geggier, Peter, Dave, Richa, Feldman, Michael B., Terry, Daniel S., Altman, Roger B., Munro, James B., Blanchard, Scott C.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 18.06.2010
• Subjects: Bacteria; Codon - genetics; Codon - metabolism; Escherichia coli - genetics; Escherichia coli - metabolism; fidelity; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Guanosine Triphosphate - chemistry; Guanosine Triphosphate - metabolism; Hydrolysis; induced fit; initial selection; Kinetics; Macromolecular Substances; Models, Biological; Models, Molecular; Nucleic Acid Conformation; Peptide Elongation Factor Tu - chemistry; Peptide Elongation Factor Tu - metabolism; Ribosomes - metabolism; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Transfer, Amino Acyl - chemistry; RNA, Transfer, Amino Acyl - metabolism; RNA, Transfer, Met - chemistry; RNA, Transfer, Met - metabolism; RNA, Transfer, Phe - chemistry; RNA, Transfer, Phe - metabolism; tRNA selection; EF-Tu; TDP; GAC; induced fit; fidelity; smFRET; aa-tRNA; SRL; CR; tRNA selection; initial selection; GA; PTC

Aminoacyl-tRNA (aa-tRNA), in a ternary complex with elongation factor-Tu and GTP, enters the aminoacyl (A) site of the ribosome via a multi-step, mRNA codon-dependent mechanism. This process gives rise to the preferential selection of cognate aa-tRNAs for each mRNA codon and, consequently, the fidel...