Engineering a growth sensor to select intracellular antibodies in the cytosol of mammalian cells

• Published in: Journal of bioscience and bioengineering Vol. 124; no. 1; pp. 125 - 132
• Main Authors: Nguyen, Thuy Duong, Takasuka, Hitoshi, Kaku, Yoshihiro, Inoue, Satoshi, Nagamune, Teruyuki, Kawahara, Masahiro
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 01.07.2017
• Subjects: Animals; antibodies; Antibodies - genetics; Antibodies - immunology; antigens; bacteriophages; c-kit; cytosol; Cytosol - metabolism; engineering; Growth signal; Intracellular antibody; Library screening; Mammalian cell; mammals; nucleoproteins; Nucleoproteins - immunology; Peptide Library; Protein Engineering - methods; Rabies lyssavirus; Rabies virus; Receptor engineering; therapeutics; Library screening; Receptor engineering; Mammalian cell; c-kit; Intracellular antibody; Growth signal; Rabies virus

Intracellular antibodies (intrabodies) are expected to function as therapeutics as well as tools for elucidating in vivo function of proteins. In this study, we propose a novel intrabody selection method in the cytosol of mammalian cells by utilizing a growth signal, induced by the interaction of th...