Cell- and Lamina-Specific Expression and Activity-Dependent Regulation of Type II Calcium/Calmodulin-Dependent Protein Kinase Isoforms in Monkey Visual Cortex

• Published in: The Journal of neuroscience Vol. 18; no. 6; pp. 2129 - 2146
• Main Authors: Tighilet, Brahim, Hashikawa, Tsutomu, Jones, Edward G
• Format: Journal Article
• Language: English
• Published: United States Soc Neuroscience 15.03.1998; Society for Neuroscience
• Subjects: Animals; Calcium-Calmodulin-Dependent Protein Kinases - genetics; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Functional Laterality - physiology; gamma-Aminobutyric Acid - metabolism; Immunohistochemistry; Isoenzymes - genetics; Isoenzymes - metabolism; Macaca; Neurons - metabolism; Ocular Physiological Phenomena; Reference Values; RNA, Messenger - metabolism; Sensory Deprivation - physiology; Tissue Distribution; Vision, Monocular - physiology; Visual Cortex - cytology; Visual Cortex - enzymology
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.18-06-02129.1998

In situ hybridization histochemistry and immunocytochemistry were used to study localization and activity-dependent regulation of α, β, γ, and δ isoforms of type II calcium/calmodulin-dependent protein kinase (CaMKII) and their mRNAs in areas 17 and 18 of normal and monocularly deprived adult macaques. CaMKII-α is expressed overall at levels three to four times higher than that of CaMKII-β and at least 15 times higher than that of CaMKII-γ and -δ. All isoforms are expressed primarily in pyramidal cells of both areas, especially those of layers II–III, IVA (in area 17), and VI, but are also expressed in nonpyramidal, non-GABAergic cells of layer IV of both areas and in interstitial neurons of the white matter. CaMKII-α and -β are colocalized, suggesting the formation of heteromers. There was no evidence of expression in neuroglial cells. Each isoform has a unique pattern of laminar and sublaminar distribution, but cortical layers or sublayers enriched for one isoform do not correlate with layers receiving inputs only from isoform-specific layers of the lateral geniculate nucleus. CaMKII-α and -β mRNA and protein levels in layer IVC of area 17 are subject to activity-dependent regulation, with brief periods of monocular deprivation caused by intraocular injections of tetrodotoxin leading to a 30% increase in CaMKII-α mRNA and a comparable decrease in CaMKII-β mRNA in deprived ocular dominance columns, especially of layer IVCβ. Expression in other layers and expression of CaMKII-γ and δ were unaffected. Changes occurring in layer IVC may influence the formation of heteromers and protect supragranular layers from CaMKII-dependent plasticity in the adult.