Transcriptome Profiling Reveals Enhanced Mitochondrial Activity as a Cold Adaptive Strategy to Hypothermia in Zebrafish Muscle

• Published in: Cells (Basel, Switzerland) Vol. 12; no. 10; p. 1366
• Main Authors: Cahill, Thomas, Chan, Sherine, Overton, Ian M, Hardiman, Gary
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.05.2023; MDPI
• Subjects: Analysis; Animals; Cold tolerance; Cosmic rays; Danio rerio; Diagnosis; DNA damage; DNA repair; Endoplasmic reticulum; Exercise; Extracellular matrix; Gene Expression Profiling; Glycolysis; Hibernation; Hypothermia; Hypoxia; Immunological tolerance; induced torpor; Inflammation; Mars; Melatonin; Microgravity; Mitochondria; muscle; Muscles; Oxidative phosphorylation; Phenotypes; Phosphorylation; Physical activity; Physical fitness; Post-irradiation; Protein synthesis; Protein turnover; Proteins; Radiation; Sarcopenia; Space flight; Stat3 protein; Temperature; Torpor; Torpor - physiology; Transcription factors; Transcriptomes; Transcriptomics; Translation; Travel; Weightlifting; Zebra fish; Zebrafish; Zebrafish - genetics; United Kingdom; United States > US; radiation; DNA damage; induced torpor; mitochondria; muscle; transcriptomics; zebrafish; hibernation
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12101366

The utilisation of synthetic torpor for interplanetary travel once seemed farfetched. However, mounting evidence points to torpor-induced protective benefits from the main hazards of space travel, namely, exposure to radiation and microgravity. To determine the radio-protective effects of an induced torpor-like state we exploited the ectothermic nature of the (zebrafish) in reducing their body temperatures to replicate the hypothermic states seen during natural torpor. We also administered melatonin as a sedative to reduce physical activity. Zebrafish were then exposed to low-dose radiation (0.3 Gy) to simulate radiation exposure on long-term space missions. Transcriptomic analysis found that radiation exposure led to an upregulation of inflammatory and immune signatures and a differentiation and regeneration phenotype driven by and transcription factors. In addition, DNA repair processes were downregulated in the muscle two days' post-irradiation. The effects of hypothermia led to an increase in mitochondrial translation including genes involved in oxidative phosphorylation and a downregulation of extracellular matrix and developmental genes. Upon radiation exposure, increases in endoplasmic reticulum stress genes were observed in a torpor+radiation group with downregulation of immune-related and ECM genes. Exposing hypothermic zebrafish to radiation also resulted in a downregulation of ECM and developmental genes however, immune/inflammatory related pathways were downregulated in contrast to that observed in the radiation only group. A cross-species comparison was performed with the muscle of hibernating (brown bear) to define shared mechanisms of cold tolerance. Shared responses show an upregulation of protein translation and metabolism of amino acids, as well as a hypoxia response with the shared downregulation of glycolysis, ECM, and developmental genes.